How the FluidFM ADD-ON brings even more "Bio" into BioAFMs

bioscope resolve, flexafm and nanowizard

Would you like to unlock unique biological applications for your AFM? Are you interested in single-cell experiments?
Then FluidFM might be the right technology to help you with your research. 

FLUIDIC FORCE MICROSCOPY – MAKING SCIENTIFIC IMPACT

Fluidic Force Microscopy (FluidFM) technology is a unique combination of microfluidics and atomic force microscopy. Each patented hollow FluidFM probe (i.e. cantilever) type unlocks a wealth of new applications for AFM users. Since its invention in 2009, FluidFM has gained the reputation of enabling unprecedented single cell biology and biophysical experiments.

Odoo CMS - a big picture

The scientific appeal of FluidFM is underlined by already more than 60 publications with an impressive average impact factor above 8. Thanks to a rapidly growing user base with systems being placed at the moment in more than 70 laboratories around the globe, we expect even more breakthroughs by the FluidFM community soon. An important reason for this rapid user growth has been our selected partnerships with the three major BioAFM manufacturers, Bruker, JPK and Nanosurf.
Now what exactly does FluidFM offer to an AFM user?


FluidFM - GO BEYOND AFM IMAGING

For BioAFM users, FluidFM unlocks many exciting applications beyond imaging; ranging from single cell gene editing,  to much faster mechanical measurements such as single cell force spectroscopy (SCFS), through to direct printing of sub-micron feature patterns.

DAPI printed on DNA coated glass

While it is not uncommon that new FluidFM users develop their unique application, popular FluidFM applications include:

Odoo CMS - a big picture
Odoo CMS - a big picture

RECENT FluidFM HIGHLIGHTS

FluidFM allows creative and unique AFM research, which results often in high impact publications; here is a selection of three publication highlights just from the past few months:

The discovery of a new adhesion mechanism for the notorious Candida Albicans pathogen.

J. Dehullu, C. Valotteau, P. Herman-Bausier, M. Garcia-Sherman, M. Mittelviefhaus, J.A. Vorholt, P.N. Lipke & Y.F. Dufrêne. Fluidic Force Microscopy Demonstrates that Homophilic Adhesion by Candida albicans Als Proteins is Mediated by Amyloid Bonds between Cells. (2019) Nano Letters. doi:10.1024/acs.nanolett.9b01010

See publication

A break-through method to detect single extra or intra-cellular ions with a modified FluidFM setup.

M. Aramesh, C. Forró, L. Dorwling-Carter, I. Lüchtefeld, T. Schlotter, S.J. Ihle, I. Shorubalko, V. Hosseini, D. Momotenko, T. Zambelli, E. Klotzsch & J. Vörös. Localized detection of ions and biomolecules with a force-controlled scanning nanopore microscope. (2019) Nature Nanotechnology. doi: 10.1038/s41565-019-0493-z

See publication

A systematic single-cell mechanical study of the leaf microbiota.

M. Mittelviehfhaus, D.B. Müller, T. Zambelli & J.A. Vorholt. A modular atomic force microscopy approach reveals a large range of hydrophobic adhesion forces among bacterial members of the leaf microbiota. (2019) The ISME Journal. doi: 10.1038/s41396-019-0404-1

See publication

THE FluidFM ADD-ON – TAILORED TO YOUR AFM

FluidFM probe for Bruker AFM systems
FluidFM probe for Bruker JPK and Nanosurf systems
FluidFM probe for the FluidFM BOT system

FluidFM probes - easy to use and tailored to each specific system. The FluidFM chip comes pre-glued to a FluidFM adapter that is tailored to a specific system. The FluidFM adapter features an integrated liquid reservoir and pneumatic connections.

A functional FluidFM probe needs a liquid reservoir, pneumatic connections and micrometer precise mounting. For this reason, we developed the FluidFM ADD-ON solutions in cooperation with the AFM manufacturers. This allowed us to carefully consider the geometrical and physical requirements of both the FluidFM parts as well as the AFM system. This results in a unique FluidFM probe holder design for selected AFMs and tailored FluidFM probes, accompanied by the state-of-the-art FluidFM microfluidics control system.

FluidFM ADD-ON system

compatible systems

FluidFM ADD-ON solutions are already available for the following AFM families:
Odoo - Sample 1 for three columns

BioScope Resolve

Odoo - Sample 2 for three columns

NanoWizard, CellHesion, ForceRobot

Odoo - Sample 3 for three columns

FlexAFM, CoreAFM

If you are interested to learn more about how you could add FluidFM to your existing AFM, we look forward to hearing from you: