HANDLING COLLOIDAL PROBES.
Spherical Colloids as Ideal Probes.
While colloidal probes are inherently difficult to produce and handle, FluidFM® technology overcomes these limitations.
Spherical colloids are the most suitable probes for local elasticity measurements on complex substrates. Interactive forces between colloidal particles and other surfaces can be measured directly with FluidFM, which offers you unparalleled flexibility for your most demanding research requirements.
PROBES PER CANTILEVER
PROBE PER MINUTE
Reversible Colloid Attachment.
The simple, yet universal approach enabled by FluidFM technology allows to reversibly attach micro- and nanospheres to an atomic force cantilever in order to function as a colloidal probe.
The colloids are seized and reversibly attached to the FluidFM probe by applying an underpressure to the microfluidic channel. Once measurement with the attached colloid concludes, it can be easily detached from the probe by application of a short overpressure puls.
Get More Insights. Faster.
Quantify long-term or irreversible interactions by using each colloidal probe only once.
Obtain solid statistics in short periods of time by measuring more data points than ever before. The versatility of FluidFM thereby allows you to use solid, liquid and gaseous colloids as required by your experiment.
Simple and economical.
Simplify your colloidal spectroscopy experiments with the use of FluidFM.
FluidFM technology makes opting for a completely fresh probe inherently easy: Imagine renewing your AFM colloidal probe in-situ without having to completely replace the entire probe. Fast, in-situ renewal of your probe is possible with FluidFM technology - at virtually no cost.
N. Helfricht, A. Mark, L. Dorwling-Carter, T. Zambelli and G. Papastavrou. Extending the Limits of Direct Force Measurements: Colloidal Probes from Sub – Micron Particles. Nanoscale. doi: 10.1039/C7NR02226C.
N. Helfricht, E. Doblhofer, J. F.L. Duval, T. Scheibel & G. Papastavrou. Colloidal Properties of Recombinant Spider Silk Protein Particles. Journal of Physical Chemistry C, 120(32), doi:10.1021/acs.jpcc.6b03957
B. R. Simona, L. Hirt, L. Demkó, T. Zambelli, J. Vörös, M. Ehrbar & V. Milleret. Density gradients at hydrogel interfaces for enhanced cell penetration. Biomater. Sci. doi:10.1039/C4BM00416G
P. Dörig, D. Ossola, A. M. Truong, M. Graf, F. Stauffer, J. Vörös & T. Zambelli.
Exchangeable colloidal AFM probes for the quantification of irreversible and long-term interactions.
Biophysical Journal, 105 (2), 463 – 472. doi:10.1016/j.bpj.2013.06.002