Single Bacteria Adhesion | Factsheet

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1. CYTOSURGE AG, SÄGEREISTRASSE 25, 8152 GLATTBRUGG, SWITZERLAND PHONE +41 44 533 14 50, FAX +41 44 533 14 59, WWW.CYTOSURGE.COM CONTACT US. We offer complete support for our customers and distributors. Please visit the Cytosurge Help Center in order to access the FluidFM ® user community. www.fluidfm.com SINGLE BACTERIA ADHESION Measuring bacterial adhesion at the single cell level is now possible thanks to FluidFM . Quantify anti- microbial surfaces, gain insight into pathogen pathways, or explore your own unique application. FluidFM technology gives you ac - cess to new realms of the microbio - logical domain. F luid FM ® GIVES YOU THE EDGE. Enjoy the unique opportunity to mea - sure the adhesion of individual bacteria. The short preparation time in combina - tion with reusable measurement probes makes FluidFM the perfect tool for all your single cell adhesion studies. THE PROCEDURE IN BRIEF. The target cell is selected via the point and click interface and then revers- ibly attached to the FluidFM probe by applying an underpressure. It is sub- sequently detached from the surface BACTERIA. An S. pyogenes cell (highlighted in blue) is detached from the surface using a FluidFM probe cantilever. The outlines of the pyramidal tip can be seen around the cell. Courtesy of Potthoff E., ETH Zurich by retracting the measurement probe, and the resulting adhesion forces are precisely recorded with pN resolution. All force curves are automatically stored and organized for analysis using our advanced data analysis software or custom third-party tools. SELECTED PUBLICATION – 2015. E. Potthoff, D. Ossola, T. Zambelli & J. A. Vorholt. Bacterial adhesion force quantification by fluidic force microscopy. (2015) Nanoscale , 7 (9), 4070 – 4079. doi:10.1039/c4nr06495j POINT & CLICK SIMPLE OPERATION pN RESOLUTION UNIQUE NO OTHER METHOD F luid FM ® – A WORLD OF APPLICATIONS. Subject to change without notice. Revision: 1.3 ID: CYTO-14-49 Copyright © 2016 Cytosurge AG, Switzerland

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