Welcome note & FluidFM announcements (15 min)

Welcome note & FluidFM announcements (15 min)

  • Pablo Doerig
  • Dec 01, 2021 09:00 AM
  • Digital

    Welcome note. Conference introduction. Advances and novelties offered by FluidFM technology and solutions....

    Single-Cell Based Research Enabled by Combined Atomic Force Microscopy and Microfluidic Delivery

    Single-Cell Based Research Enabled by Combined Atomic Force Microscopy and Microfluidic Delivery

    • Gang-Yu Liu
    • Dec 01, 2021 09:15 AM
    • Digital

      Combining microfluidic probe with atomic force microscopy (AFM) developed by Cytosurge AG, researchers are able to probe and investigate chemical and biological events occurring at single cell level.  Single cell mechanics, derived from atomic force microscopy-based technology in conjunction with cellular imaging and assays, provides a new and effective means to measure the elastic compliance of cellular membrane, cytoskeleton, as well as nuclei.  In addition, applying mechanical force to living...

      Spotting of 2D viral structures by FluidFM

      Spotting of 2D viral structures by FluidFM

      • Christine Müller-Renno
      • Dec 01, 2021 09:35 AM
      • Digital

        Nanotechnology plays an important role in different areas of application. Because top-down based fabrication technologies are restricted by physical and technical limits, bottom-up based fabrication technologies are of increasing interest. In this context, small building blocks are used to build larger objects through self-assembly. Because of their size and properties, plant viruses are ideal building blocks. Here, tomato bushy stunt viruses (TBSV), icosahedral plant viruses of 30 nm diameter, ...

        10 min break

        • Dec 01, 2021 09:55 AM

          ...

          Novel applications of FluidFM OMNIUM in combination with high resolution label-free biosensors

          Novel applications of FluidFM OMNIUM in combination with high resolution label-free biosensors

          • Kinga Dóra Kovács
          • Dec 01, 2021 10:05 AM
          • Digital

            The present talk shortly introduces the main research activity of the Nanobiosensorics Laboratory (Budapest, Hungary, https://nanobiosensorics.com) especially in relation to fluidic force microscopy. We mainly focus on label-free techniques for cell biological and biophysical research and on developing kinetic biophysical models (Kanyo et al., 2020; Saftics et al., 2021). Recently by employing resonant waveguide gratings, we could demonstrate single-cell resolution and measure the adhesion kinet...

            Probing the interactions between air bubbles and (bio)-interfaces at the molecular scale using FluidFM

            Probing the interactions between air bubbles and (bio)-interfaces at the molecular scale using FluidFM

            • Cécile Formosa-Dague
            • Dec 01, 2021 10:25 AM
            • Digital

              Understanding the molecular mechanisms underlying bubble-(bio)surfaces interactions is currently a challenge that if overcame, would allow to understand and control the various processes in which they are involved. Atomic force microscopy is a valuable tool to measure such interactions, but it is limited by the large size and instability of bubbles that can be attached on surfaces or on AFM cantilevers. To overcome these challenges, we here develop a new method to probe more accurately the inter...

              4D force detection of cell adhesion and contractility combining FluidFM and confocal reference-free TFM

              4D force detection of cell adhesion and contractility combining FluidFM and confocal reference-free TFM

              • Xinyu Zhang
              • Dec 01, 2021 10:45 AM
              • Digital

                Fluid Force Microscopy (FluidFM) combines a hollow microchannel cantilever with conventional AFM and has proven to be a promising tool in biological applications, especially in cell mechanics. [1] FluidFM and Atomic Force Microscopy (AFM) provide abundant force sensitivity in the perpendicular direction but lack information in the xy lateral direction. Confocal reference-free Traction force microscopy (cTFM) is a powerful tool for the quantification of cellular forces and allows the continuous h...

                10 min break

                • Dec 01, 2021 11:05 AM

                  ...

                  Mass measurements on beads, cells and cellular compartments with FluidFM

                  Mass measurements on beads, cells and cellular compartments with FluidFM

                  • Hans Gunstheimer
                  • Dec 01, 2021 11:15 AM
                  • Digital

                    In this presentation we will discuss mass measurements by FluidFM ® Micropipettes, their accuracy and limits illustrated with examples from biology and material science. Mass as a fundamental property gives us valuable information about the condition of a measurement object. Taking a single biological cell for example, the dysregulation of its mass and its mass development can be related to many diseases (1). To relate the mass of the cell to its condition it is favorable to combine it with othe...

                    Is the spermatozoon swimming force essential for fertilization?

                    Is the spermatozoon swimming force essential for fertilization?

                    • Alice Battistella
                    • Dec 01, 2021 11:35 AM
                    • Digital

                      In mammalian fertilization process, one, and only one, spermatozoon must cross the glycoprotein thick protective shell, the zona pellucida (ZP) and enter the oocyte to initiate the meiotic division. We recently demonstrated that the mechanics of this layer plays an important role in the process and show that during ageing the ZP nearly double its stiffness thus reducing the probability of fertilization (Battistella 2021). This suggests that, beside the enzymatic mechanism, also the mechanical in...

                      Closing note

                      Closing note

                      • Pablo Doerig
                      • Dec 01, 2021 11:55 AM
                      • Digital

                        ...

                        Round table: Single cell omics (75 min)

                        • Dec 01, 2021 02:30 PM
                        • Digital

                        Interactive round table to discuss approaches, ideas, and solutions related to FluidFM research in single cell omics....

                        Round table: Nanoprinting (75 min)

                        • Dec 01, 2021 04:00 PM
                        • Digital

                        Interactive round table to discuss approaches, ideas, and solutions related to FluidFM research in nanoprinting....

                        Round table: Neuroscience (75 min)

                        • Dec 02, 2021 09:00 AM
                        • Digital

                        Interactive round table to discuss approaches, ideas, and solutions related to FluidFM research in neuroscience....

                        Round table: Mechanobiology (75 min)

                        • Dec 02, 2021 10:30 AM
                        • Digital

                        Interactive round table to discuss approaches, ideas, and solutions related to FluidFM research in mechanobiology....

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