Single-Cell Based Research Enabled by Combined Atomic Force Microscopy and Microfluidic Delivery

Proposed by Gang-Yu Liu

Combining microfluidic probe with atomic force microscopy (AFM) developed by Cytosurge AG, researchers are able to probe and investigate chemical and biological events occurring at single cell level.  Single cell mechanics, derived from atomic force microscopy-based technology in conjunction with cellular imaging and assays, provides a new and effective means to measure the elastic compliance of cellular membrane, cytoskeleton, as well as nuclei.  In addition, applying mechanical force to living cells could actively trigger and regulate cellular signaling processes and ultimately allow regulation of cellular behaviors. This presentation first introduces the technology (Figure 1), methodology, and latest developments to perform single cell mechanics measurements in vitro, and to extract the cellular mechano-profiles qualitatively and quantitatively. The presentation will also share technical challenges in the pursuit of single-cell-based research in the hope to work with peers to address these challenges and explore new and potential applications.

Figure 1. Schematic diagram of single cell mechanics measurements.

Gang-Yu Liu, Evgeny Ogorodnik, Arpad Karsai, Department of Chemistry and Biophysics Graduate Group, University of California, Davis, CA 95616, United States.

About The Speaker

UC Davis Liu Group, Gang-Yu Liu

Gang-Yu Liu

Professor, Dept. of Chemistry, UC Davis, USA

Gang-yu Liu received her Ph.D. from Princeton University in 1992, and completed her postdoctoral research at UC Berkeley in 1994 as a Miller Research Fellowship. She has received several prestigious awards, including an ACS Fellow in 2010, AAAS Fellow in 2007, Sloan Faculty Recognition Award in 2007, NSF-CAREER Award (1997), Arnold and Mable Beckman Young Investigator Award (1996-1998), and the Camille and Henry Dreyfus New Faculty Award (1994-1999). She has been a senior editor for the J. Phys. Chem. since 2005. She serves on editorial advisory boards for ACS Nano, (2007-Present) and the Annual Rev. of Phys. Chem. (2010-present). Liu’s overall research objectives focus on the development of advanced nanotechnology and exploration of its applications in materials science and biomedicine. One important aspect of the research is the design and engineering nanostructures which position reaction sites on surfaces with high precision. The exquisite precision attainable by the nanolithographic methods they have developed allows complex multivalent interactions such as surface reactions and bio-recognition to be controlled by varying the separation and local environment of the binding sites. 

Practical Info

Dec 01, 2021 09:15 AM (Europe/Zurich)
20 minutes