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In this protocol, we use the Sigmacote® solution from Sigma-Aldrich®. This solution of silicone in heptane will form a hydrophobic microscopic thin film on the surface of the probe.
Warning: Sigmacote chemicals present several hazard statements. Handling of Sigmacote therefore requires some safety precautions, according to the safety data sheet provided by the supplier.
- A desiccator with a vacuum pump system
- Sigmacote® solution (Sigma® SL2-25ML). Please refer to sigmacote product information sheet for more information.
- The Cytosurge® probe
- A container for the probe (Here we use 12 well plates)
- Disposable wipers
- Plastic transferpipette
- Prepare the desiccator with one disposable wiper and a container for the probe.
- Place the Cytosurge® probe upside down inside the container.
- Pipette 1-2 drop of Sigmacote® onto the bottom surface of the desiccator.
- Apply vacuum for 1h.
At this step, reaching an absolute pressure of +200mbar inside of the desiccator is optimal for the coating.
- For an optimal coating, heat the probe at 80°C for 1h in a dry oven.
- The probe is now ready to use for further experiments, and can be stored up to one week. If not using the probe within one week, repeat the whole procedure for optimal performance.
Depending on the environmental conditions (humidity, temperature), condensation might occur when releasing the pressure by opening the desiccator, or in the oven. Adding desiccants both in the oven and the desiccator should solve this issue.
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|Asked: 11/20/18, 7:05 AM|
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|Last updated: 11/20/18, 7:06 AM|