Welcome to the fluidfm forum

This community is for professionals and enthusiasts of our products and services. Share and discuss the best applications, experiments and new ideas, build your professional profile and become a better researcher together.

Please read the guidelines before participating in this community.

0

Demo protocol: Glycerol spotting on glass

By
Pablo Doerig
on 8/31/18, 2:58 AM 309 views

How can I perform glycerol spotting on glass with FluidFM?

This question has been included in the official documentation.

0

Pablo Doerig

--Pablo Doerig--

1048
| 3 2 2
Glattbrugg, Switzerland
--Pablo Doerig--

Dr., VP AFM Solutions and Head Customer Success, Cytosurge AG, Switzerland

Pablo Doerig
On 8/31/18, 3:04 AM

A smooth start into the world of FluidFM

This protocol aims to both show functionality of the FluidFM setup and provides inexperienced users with a convenient and easy start into the world of FluidFM. Because glycerol does not evaporate, the most common cause to end a probe lifetime is excluded: drying out.

Using glycerol in a nanopipette, this protocol will allow the user to print micron-sized spots on any glass-surface, in air. The spot will be visible with 20x magnification or larger.

INK

Glycerol, pure

(Optional: Mix with a fluorescent tracer of choice)


PROBE

FluidFM Nanopipette, 2 N/m


SAMPLE CONTAINER

Glass bottom container or glass slide


PREPARE FLUIDFM SETUP

  • Fill probe with ink

  • Mount probe

  • Align the laser and optimize the signal

  • Fill the probe

  • Apply 1000 mbar until glycerol reaches probe aperture

  • The glycerol will not exit the probe at this pressure

  • Apply 20 mbar pressure to prevent air coming in from the front

  • Go to the sample

  • Approach to the surface

  • Retract 20 um from surface


SPOTTING A PATTERN

  • Use an idle pressure of 0 mbar between two spots

  • Spot the pattern of your choice with following parameters:

  • 0-20 mbar will result in tiny spots (<1 um)

  • 20-100 mbar will result in ~ 5 um spots

  • Setpoint 20 nN o Contact time 300 ms

  • Approach speed 10 um/s

  • Retract speed 30 um/s

  • Retract distance 10 um


In the image, the glycerol was mixed with a fluorescent tracer for maximum visibility


AFTER THE EXPERIMENT

  • The probe can be stored up to several days in air

  • A dark, dry and cool environment is recommended.

  • Flag

Your Answer

Please try to give a substantial answer. If you wanted to comment on the question or answer, just use the commenting tool. Please remember that you can always revise your answers - no need to answer the same question twice. Also, please don't forget to vote - it really helps to select the best questions and answers!

Keep Informed

About This Community

This community is for professionals and enthusiasts of our products and services. Share and discuss the best applications, experiments and new ideas, build your professional profile and become a better researcher together. Read Guidelines

Question tools

1 follower(s)

Stats

Asked: 8/31/18, 2:58 AM
Seen: 309 times
Last updated: 5/14/19, 11:29 AM

You want to see the most relevant discussions from the forum structured per topic?