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Melanie Schwabe

--Melanie Schwabe--

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Glattbrugg, Switzerland
--Melanie Schwabe--


Melanie Schwabe
On 7/4/18, 11:53 AM


The filling procedure varies slightly depending on the FluidFM product you purchased. Please follow the instructions for FluidFM BIO or AFM Series after reading the general remarks.

General remarks

Filtering: FluidFM probes feature a microchannel and often a sub-micrometer aperture that can easily be clogged by any kind of particle. To avoid precipitates or any particle in your solution, always filter them through maximum 0.2 µm pore sizes before their use.

Degassing: Degassing the liquid before filling will minimize bubbles in the microchannel, especially for applications with negative pressure (e.g. adhesion).

Probe manipulation: Wear gloves during the filling operation to minimize contamination and dirt on the probe. Also, be careful to keep the upper border of the reservoir clean. Any presence of liquid may be deposited on the sealing screw of the FluidFM BIO Series system head. This could lead to cross contamination and air tightness failure. In case of contamination, please refer to cleaning of the sealing screw.

Fluorescent tracer (optional): A fluorescent tracer, such as FITC or Lucifer Yellow, can be added to the solution to later monitor the probe filling progress using epi-fluorescence. This can be very valuable especially for inexperienced user.


FluidFM BIO Series probes

The following steps describe how a FluidFM probe is best prepared:

  • Using a 1µl pipette, aspirate 0.5 µl to 1µl of the solution you would like to load the FluidFM probe with.

  • Wipe any deposit of solution off the pipette tip to avoid contaminating the outside the FluidFM probe’s reservoir.

  • Insert the pipette tip until you reach the bottom of the reservoir and eject the solution there.

                                            
                                 

  • Gently shake the FluidFM probe to be sure that the solution sits at the bottom of the FluidFM probe’s reservoir.

  • Place probe into the FluidFM loading plate and pick the probe up as soon as possible to avoid evaporation of your reservoir liquid.

FluidFM AFM Series probes

  • Fix the FluidFM probe onto the probe holder.

  • Using a 10 µl pipette, aspirate the solution you would like to load the FluidFM probe with:

    • FlexAFM and Nanowizard: Use 5 µl.

    • BioscopeResolve: Use 1 µl.

  • Wipe any deposit of solution off the pipette tip to avoid contaminating the outside the FluidFM probe’s reservoir.

  • Insert the pipette tip until you reach the bottom of the reservoir and eject the liquid there.

  • Gently shake the FluidFM probe to be sure that the solution sits at the bottom of the FluidFM probe’s reservoir.

  • For FlexAFM and Nanowizard:

    • Link the pneumatic connector to the FluidFM probe.

    • Place the magnetic probe holder onto the AFM.

    • Plug the pneumatic connector to the AFM.

    • Place the AFM on the inverted microscope.

    • Connect the pressure source to the AFM.

  • For BioScope resolve:

    • Mount the probe holder onto the AFM.

    • Place the AFM onto the inverted microscope.

    • Connect the pneumatic tubings.

  • Observe the probe in air through the microscope.



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Asked: 7/4/18, 11:34 AM
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Last updated: 11/27/19, 3:07 PM