Welcome to the fluidfm forum

This community is for professionals and enthusiasts of our products and services. Share and discuss the best applications, experiments and new ideas, build your professional profile and become a better researcher together.

Please read the guidelines before participating in this community.

1

Filling the reservoir of a FluidFM probe

By
Melanie Schwabe
on 7/4/18, 10:34 AM 496 views

What do I need to take into account to fill FluidFM probes correctly?

This question has been included in the official documentation.

1

Melanie Schwabe

--Melanie Schwabe--

5350
| 4 2 2
Glattbrugg, Switzerland
--Melanie Schwabe--


Melanie Schwabe
On 7/4/18, 10:53 AM


The filling procedure varies slightly depending on the FluidFM product you purchased. Please follow the instructions for FluidFM BOT or FluidFM ADD-ON after reading the general remarks.

General remarks

Filtering: FluidFM probes feature a microchannel and often a sub-micrometer aperture that can easily be clogged by any kind of particle. To avoid precipitates or any particle in your solution, always filter them through maximum 0.2 µm pore sizes before their use.

Degassing: Degassing the liquid before filling will minimize bubbles in the microchannel, especially for applications with negative pressure (e.g. adhesion).

Probe manipulation: Wear gloves during the filling operation to minimize contamination and dirt on the probe. Also, be careful to keep the upper border of the reservoir clean. Any presence of liquid may be deposited on the sealing screw of the BOT head. This could lead to cross contamination and air tightness failure. In case of contamination, please refer to cleaning of the sealing screw.

Fluorescent tracer (optional): A fluorescent tracer, such as FITC or Lucifer Yellow, can be added to the solution to later monitor the probe filling progress using epi-fluorescence. This can be very valuable especially for inexperienced user.


FluidFM BOT probes

The following steps describe how a FluidFM BOT probe is best prepared:

  • Using a 1µl pipette, aspirate 0.5 µl to 1µl of the solution you would like to load the FluidFM probe with.

  • Wipe any deposit of solution off the pipette tip to avoid contaminating the outside the FluidFM probe’s reservoir.

  • Insert the pipette tip until you reach the bottom of the reservoir and eject the solution there.

                                            
                                 

  • Gently shake the FluidFM probe to be sure that the solution sits at the bottom of the FluidFM probe’s reservoir.

  • Place probe into the BOT’s loading plate and pick the probe up as soon as possible to avoid evaporation of your reservoir liquid.

FluidFM ADD-ON probes

  • Fix the FluidFM probe onto the probe holder.

  • Using a 10 µl pipette, aspirate the solution you would like to load the FluidFM probe with:

    • FlexAFM and Nanowizard: Use 5 µl.

    • BioscopeResolve: Use 1 µl.

  • Wipe any deposit of solution off the pipette tip to avoid contaminating the outside the FluidFM probe’s reservoir.

  • Insert the pipette tip until you reach the bottom of the reservoir and eject the liquid there.

  • Gently shake the FluidFM probe to be sure that the solution sits at the bottom of the FluidFM probe’s reservoir.

  • For FlexAFM and Nanowizard:

    • Link the pneumatic connector to the FluidFM probe.

    • Place the magnetic probe holder onto the AFM.

    • Plug the pneumatic connector to the AFM.

    • Place the AFM on the inverted microscope.

    • Connect the pressure source to the AFM.

  • For BioScope resolve:

    • Mount the probe holder onto the AFM.

    • Place the AFM onto the inverted microscope.

    • Connect the pneumatic tubings.

  • Observe the probe in air through the microscope.


  • Flag

Your Answer

Please try to give a substantial answer. If you wanted to comment on the question or answer, just use the commenting tool. Please remember that you can always revise your answers - no need to answer the same question twice. Also, please don't forget to vote - it really helps to select the best questions and answers!

Keep Informed

About This Community

This community is for professionals and enthusiasts of our products and services. Share and discuss the best applications, experiments and new ideas, build your professional profile and become a better researcher together. Read Guidelines

Question tools

2 follower(s)

Stats

Asked: 7/4/18, 10:34 AM
Seen: 496 times
Last updated: 6/26/19, 7:58 AM

You want to see the most relevant discussions from the forum structured per topic?