Welcome to the fluidfm forum
This community is for professionals and enthusiasts of our products and services. Share and discuss the best applications, experiments and new ideas, build your professional profile and become a better researcher together.
Please read the guidelines before participating in this community.
Get the ideal tool for your
nano-manipulation task from our selection of FluidFM probes.
Explore the FluidFM Probes pages.
In this protocol, the FluidFM probe is coated with PAcrAm (SuSoS AG). This compound is a polymer used as antifouling agent for glass and polymer cell culture substrates. This coating is ideal for coating micropipettes in isolation workflows, but it also can be used for nanosyringe coating for the injection of specific compounds.
PAcrAm is distributed in 0.1 mg lyophilized aliquots, which also contain the buffer required for surface modification.
There are two different protocols for probe coating using PAcrAm, depending if they are coated during the experiment or before.
a. COATING WITH PAcrAm inside FluidFM system
This is ideal if you want the coating to be very fresh before starting an experiment. Only 1 probe can be coated like this at a time.
b. Pre-COATING Probes WITH PAcrAm
This protocol allows you to coat multiple probes the day before the experiment, allowing shorter setup time and higher throughput during the experiment.
We have joined forces with Harvard's Wyss Institute.
Collaboration to improve CRISPR-based multiplexed gene editing.
About This Community
|Asked: 11/7/19, 5:35 AM|
|Seen: 373 times|
|Last updated: 6/10/20, 3:53 AM|