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In this protocol, the FluidFM probe is coated with PAcrAm (SuSoS AG). This compound is a polymer used as antifouling agent for glass and polymer cell culture substrates. This coating is ideal for coating micropipettes in isolation workflows, but it also can be used for nanosyringe coating for the injection of specific compounds.
PAcrAm is distributed in 0.1 mg lyophilized aliquots, which also contain the buffer required for surface modification.
There are two different protocols for probe coating using PAcrAm, depending if they are coated during the experiment or before.
a. COATING WITH PAcrAm inside FluidFM system
This is ideal if you want the coating to be very fresh before starting an experiment. Only 1 probe can be coated like this at a time.
b. Pre-COATING Probes WITH PAcrAm
This protocol allows you to coat multiple probes the day before the experiment, allowing shorter setup time and higher throughput during the experiment.
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Collaboration to improve CRISPR-based multiplexed gene editing.
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|Asked: 11/7/19, 5:35 AM|
|Seen: 467 times|
|Last updated: 6/10/20, 3:53 AM|