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Once the probe has been picked up by the system and the Preparation workflow has already started, the FluidFM probe can be filled with the solution loaded previously into the reservoir.
Fill probe is one of the steps of the Preparation workflow. There are several commands, which allow the user to control the pressure applied (Fill / Idle), as well as a difference image to detect differences.
Typically, a pressure of 300-500 mbar should be applied (press Fill, with the selected pressure). Higher pressure volumes can also be used, depending on the solution loaded. The process can last from few seconds up to few minutes: it will depend on the properties of the solution loaded, as well as in the characteristics of the chosen probe. Once the probe is filled, the pressure can be changed back to Idle (3-5mbar)
How can I detect if the probe is already filled?
on the characteristics of the solution the way of visualizing the process can
be different: for fluorescent samples, the filling can be easily followed by
observing the cantilever with the respective filter set. For non-fluorescent
samples, the process can be tracked by checking the appearance of the pyramid
in the cantilever during the process and by using the difference image.
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|Asked: 6/26/19, 8:14 AM|
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|Last updated: 11/27/19, 3:07 PM|