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When using the Observation workflow in ARYA, we can get time-lapse images of selected points (e.g. cells). Together with ImageJ a time-lapse video can be generated for any observed point. In the example below, we generate a video of CHO cells injected with Lucifer Yellow and Alexa 568. In the experiment, a picture of all injected cells was taken every hour during 24h after the injection, in bright field and two fluorescence channels (green and red). A merged image of the 3 channels is also generated by default in ARYA.
The protocol can be also found in the next file:
EXPORTING DATA OF A SPECIFIC POINT WITH ARYA
Once the Observation workflow is complete, click on "Show results" (folder icon, on the bottom left corner of ARYA, indicated in red):
From the 4 icons, select the one on the right, which will group the images according to points. Select a point of interest: images will appear chronologically in the menu below. Click then in "Export point data...".
The following menu will appear. The final folder where the pictures will be stored must be specified: this folder must contain ONLY the exported pictures (unselect "create subdirectories" and "export meta data as CSV" options). Click on "Export".
CREATING A TIME-LAPSE WITH IMAGE J
Download and install ImageJ (http://imagej.nih.gov/ij/download.html).
In our example, the images are stored in the folder 320 (located in the desktop).
In ImageJ, import the images of interest by selecting File>Import>Image Sequence: