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Parameters for injecting various cell lines

By
Maria Milla
on 4/29/19, 9:04 AM 130 views


Many types of compounds can be injected into adhered cells using the FluidFM® technology. The following conditions have been determined by injecting Lucifer Yellow into different cell lines with a FluidFM BOT and resulted in high cell viability. The values can be used as a starting point for injection workflows, and are meant to be adjusted for each cell line and injected solution.

Cell line Setpoint* (mV) Approach Speed (µm/s) Retraction Speed  (µm/s) Pressure (mbar) Injection Time (s) Retraction Distance (µm) Idle Pressure (mbar)
CHO 600 50 5 30 10 20 5
HeLa 600 50 5 30 10 20 5
HEK 600 50 5 30 10 20 5
C2C12 600 50 5 30 10 20 5
MEFs 600 50 5 30 10 20 5
hESC 40 10 5 10 2 20 5
mESC 40 10 5 10 2 20 5
HepG2 40 10 5 10 10 20 5
U2OS 40 10 5 10 5 20 5
Pig neurons 600 50 5 70 10 20 5
Mouse primary hepatocytes 600 50 5 70 10 20 5
MCF 7 
500 50 5 50 5 20 5

 

*Setpoint is shown in mV for FluidFM BOT. 600mV is equivalent to ca. 1µN. 


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Asked: 4/29/19, 9:04 AM
Seen: 130 times
Last updated: 5/14/19, 11:21 AM

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