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Sterilizing the sample for FluidFM experiments

FAQ
By
Maria Milla
on 3/20/19, 10:08 AM 98 views


It is important to use sterile sample containers, even for non-biological experiments. Otherwise bacteria can create a biofilm which can clog the FluidFM cantilever and interfere in your experiments. 

Use sterilized consumables (e.g. plates and dishes). In the case of non-sterile consumables, they should be sterilized for example by autoclaving them. As an alternative, you can properly clean them with ethanol. 

A good cleaning protocol for FluidFM experiments is described below (from Guillaume-Gentil et al., 2014):

1. Clean dishes with 2-propanol. 

2. Rinse them with filtered ddH2O. 

3. Dry them with a nitrogen stream. 

4. Plasma-treat them for 2 min. 

5. The dish is now ready for FluidFM experiments. 


References

Guillaume-Gentil, O., Zambelli, T. & Vorholt, J. (2014). Isolation of single mammalian cells from adherent cultures by fluidic force microscopy. Lab on a Chip, 14 (2), 402-414. doi: 10.1039/c3lc51174j



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Asked: 3/20/19, 10:08 AM
Seen: 98 times
Last updated: 5/14/19, 11:33 AM

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