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After gripping the probe, the automated air tightness test failed or gave a warning.
What shall I do now?
The leak flow is indicated in Pascal [Pa] because any leak flow generates a pressure drop along the pneumatic tubing, which we can measure with our pressure sensors. In a perfectly tight system the air flow should be 0 ul/s and the corresponding pressure drop is also 0 Pa.
Values below 1 Pa are still safe to work, despite the warning by the system. Continue with your experiment as usual.
In case the leak generates a pressure drop above 1 Pa, troubleshooting is required:
Gripping: Drop and grip the probe again. Often this resolves the issue.
Gripping height calibration: If you can see that gripping looks "odd" and the probe does not come into the center, please calibrate the Z stage level again. Also calibrate the XY position of the Z stage again.
Tubing: Check if the tubing is connected properly and/or if there are visible damages in the tubing.
Dirty head: The sealing screw and/or prism are dirty or the O-ring is damaged. Please follow the instructions here.
Probe defect: In rare cases the consumable might have a defect if the above methods didn’t solve the problem. Have a look under the stereoscope onto the probe reservoir if you see any cracks or chipped parts please contact your FluidFM distributor sending a detailed written description of the problem.
More information is available in the latest version of ARYA (when operating the BOT, press F1 to access directly to it).
Collaboration to improve CRISPR-based multiplexed gene editing.
