Help

Welcome to the fluidfm forum

This community is for professionals and enthusiasts of our products and services. Share and discuss the best applications, experiments and new ideas, build your professional profile and become a better researcher together.

Please read the guidelines before participating in this community.

0

How to create a time-lapse video with observation data?

Avatar
Maria Milla


We have joined forces with Harvard's Wyss Institute.

Collaboration to improve CRISPR-based multiplexed gene editing.


When using the Observation workflow in ARYA, we can get time-lapse images of selected points (e.g. cells). Together with ImageJ a time-lapse video can be generated for any observed point. In the example below, we generate a video of CHO cells injected with Lucifer Yellow and Alexa 568. In the experiment, a picture of all injected cells was taken every hour during 24h after the injection, in bright field and two fluorescence channels (green and red). A merged image of the 3 channels is also generated by default in ARYA.

The protocol can be also found in the next file:



EXPORTING DATA OF A SPECIFIC POINT WITH ARYA

Once the Observation workflow is complete, click on "Show results" (folder icon, on the bottom left corner of ARYA, indicated in red):



From the 4 icons, select the one on the right, which will group the images according to points. Select a point of interest: images will appear chronologically in the menu below. Click then in "Export point data...".