Help

Welcome to the FluidFM forum

This community is for professionals and enthusiasts of our products and services. Share and discuss the best applications, experiments and new ideas, build your professional profile and become a better researcher together.

Please read the guidelines before participating in this community.

2

Parameters for injecting various cell lines

Avatar
Maria Milla


Many types of compounds can be injected into adhered cells using the FluidFM® technology. The following conditions have been determined by injecting Lucifer Yellow into different cell lines with a FluidFM system and resulted in high cell viability. The values can be used as a starting point for injection workflows, and are meant to be adjusted for each cell line and injected solution.

Cell lineSetpoint* (mV)Approach Speed (µm/s)Retraction Speed  (µm/s)Pressure (mbar)Injection Time (s)Retraction Distance (µm)Idle Pressure (mbar)
CHO6005053010205
HeLa6005053010205
HEK6005053010205
C2C126005053010205
MEFs6005053010205
hESC40105102205
mESC40105102205
HepG2401051010205
U2OS40105105205
Pig neurons6005057010205
Mouse primary hepatocytes6005057010205
MCF 7 
500505505205

 

*Setpoint is shown in mV for FluidFM OMNIUM. 600mV is equivalent to ca. 1µN. 


Avatar
Discard