Help

Welcome to the fluidfm forum

This community is for professionals and enthusiasts of our products and services. Share and discuss the best applications, experiments and new ideas, build your professional profile and become a better researcher together.

Please read the guidelines before participating in this community.

0

Print from file (BIO Series)

Avatar
Maria Milla

Cutting-edge systems for single cell research – from single cell gene editing to drug research and toxicology.  

Explore all FluidFM applications.


With the ARYA 2.1.0 version (and newer versions), it is possible to print a 2D predefined pattern, by determining specific coordinates in a .txt file. The FluidFM BIO Series system will print lines between each given point.


File creation

The file is read as a list of instructions separated by newlines. When a line starts with #, it will be skipped. The possible instructions are:

Instruction
Description
Format
ApproachThe system lowers the probe until the surface is reached, keeping the setpoint indicated in the parameters. 
approach,v,p
Retract
The system retracts the probe from the surface. 
retract,dz,v,p
In-contact move
Indicates the target position to move while keeping the selected setpoint.
ffmove,x,y,p
In-contact delay
Indicates for how long the z-position is adjusted to keep the set-point. 
ffdelay,t,p
Move
Indicates a target position to move, with x,y,z coordinates (relative to the stage position when the printing started). 
linmove,x,y,z,v,p
Delay
Indicates for how long the z-position is kept. 
delay,t,p
v is indicated in micrometer per second; p in millibar; t in seconds; x, y, z and dz in micrometers. 
** In-contact operations are only supported between approach and retract. The file must leave the system in a retracted state. 

*** The file must be in .txt format. 


Printing

In the menu, select the workflow Print from File


Click on Select File and upload the created file. Once selected, the file can be previewed: the software will overlay the file content onto the video feed, adjusting it to the current magnification. Since the preview is centered, the position of the final print might not correspond to the actual placement of the printed structure. 

After indicating the Start Position and the Setpoint, click Ok and the system will start printing. The software will show the current run instruction, as well as its line number. On every run, the file is loaded and analyzed. The run will not start in case inconsistencies are detected. Once it is finished, the initial xy position will be restored and the pressure will return to idle.    


File example

For printing the rectangle with two nested crosses (showed in the picture below), the respective file should created: 

#rectangle
approach,5,0
ffmove,20,0,50,20
ffmove,20,20,50,20
ffmove,0,20,50,20
ffmove,0,0,50,20
retract,10,10,0
# cross a
linmove,10,0,10,10,0
approach,5,0
ffmove,10,20,50,20
retract,10,10,0
linmove,0,10,10,10,0
approach,5,0
ffmove,20,10,50,20
retract,10,5,10
# cross b
linmove,5,0,10,10,0
approach,5,0
ffmove,5,10,50,20
retract,10,10,0
linmove,0,5,10,10,0
approach,5,0
ffmove,10,5,50,20
retract,10,5,10


Find more information in manual of the latest ARYA version

We have joined forces with Harvard's Wyss Institute.

Collaboration to improve CRISPR-based multiplexed gene editing.


Avatar
Discard