Help

Welcome to the FluidFM forum

This community is for professionals and enthusiasts of our products and services. Share and discuss the best applications, experiments and new ideas, build your professional profile and become a better researcher together.

Please read the guidelines before participating in this community.

0

Sterilizing the sample for FluidFM experiments

Avatar
Maria Milla


It is important to use sterile sample containers, even for non-biological experiments. Otherwise, bacteria can create a biofilm that can clog the FluidFM cantilever and interfere in your experiments. 

Use sterilized consumables (e.g. plates and dishes). In the case of non-sterile consumables, they should be sterilized for example by autoclaving them. As an alternative, you can properly clean them with ethanol. 

A good cleaning protocol for FluidFM experiments is described below (from Guillaume-Gentil et al., 2014):

1. Clean dishes with 2-propanol. 

2. Rinse them with filtered ddH2O. 

3. Dry them with a nitrogen stream. 

4. Plasma-treat them for 2 min. 

5. The dish is now ready for FluidFM experiments. 


References

Guillaume-Gentil, O., Zambelli, T. & Vorholt, J. (2014). Isolation of single mammalian cells from adherent cultures by fluidic force microscopy. Lab on a Chip, 14 (2), 402-414. doi: 10.1039/c3lc51174j



Avatar
Discard