Cleaning the probe between cell adhesion measurements

To remove cell debris from the FluidFM probe, a cleaning step is necessary between two measurements of mammalian cells. The following cleaning solutions are recommended:

  • Bleach (5% Sodium Hypochlorite): Bleach cleans the probe very fast and thoroughly. It has to be handled with care as it can corrode your equipment. 

  • Terg-a-zyme: Enzyme-based cleaning solution. Slower than bleach, but gentle to the equipment. 

The cleaning can be performed as follows: Cleaning solution - water - water - medium - back to cells

  • Leave the probe on the system: Instead of moving the probe exchange the dishes below the probe and di. 

  • Cleaning dip: 5 sec to 60 sec in bleach, or at least 30 sec in Terg-a-zyme. Avoid bleach contact with the FluidFM pneumatic connector, as it can degrade the O-Ring, causing leaks. 

  • Water dip 2x: Dip the probe twice in filtered ddH20 water. In two different dishes to remove any bleach. 

  • Medium dip: Dip the probe in medium before going back to the sample with the cells. To avoid osmotic shocks.

  • Renew the coating: Protein coatings are degraded by these cleaning steps. Renew the coating if applicable. 

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