Cleaning the probe between cell adhesion measurements
To remove cell debris from the FluidFM probe, a cleaning step is necessary between two measurements of mammalian cells. The following cleaning solutions are recommended:
Bleach (5% Sodium Hypochlorite): Bleach cleans the probe very fast and thoroughly. It has to be handled with care as it can corrode your equipment.
Terg-a-zyme: Enzyme-based cleaning solution. Slower than bleach, but gentle to the equipment.
The cleaning can be performed as follows: Cleaning solution - water - water - medium - back to cells
Leave the probe on the system: Instead of moving the probe exchange the dishes below the probe and di.
Cleaning dip: 5 sec to 60 sec in bleach, or at least 30 sec in Terg-a-zyme. Avoid bleach contact with the FluidFM pneumatic connector, as it can degrade the O-Ring, causing leaks.
Water dip 2x: Dip the probe twice in filtered ddH20 water. In two different dishes to remove any bleach.
Medium dip: Dip the probe in medium before going back to the sample with the cells. To avoid osmotic shocks.
Renew the coating: Protein coatings are degraded by these cleaning steps. Renew the coating if applicable.