Single Cell Force Spectroscopy with FluidFM
Please find below an example of a quantification of adhesion forces between yeast and mammalian cells using FluidFM technology. This video is based on a protocol developed at ETH Zürich in the laboratory of Prof. Julia Vorholt.
Potthoff, E., Guillaume-Gentil, O., Ossola, D., Polesel-Maris, J., LeibundGut-Landmann, S., Zambelli, T. & Vorholt, J.A. Rapid and Serial Quantification of AdhesionForces of Yeast and Mammalian Cells (2012), PloS ONE, 7(12): e52712. doi:10.1371/journal.pone.0052712
Potthoff, E., Franco, D., D'Alessandro, V., Starck, C., Falk, V., Zambelli, T., Vorholt, J.A., Poulikakos, D. & Ferrari, A. Toward a Rational Design of Surface Textures Promoting Endothelialization (2014), Nano Letters, 14(2), pp 1069-1079. doi:10.1021/nl4047398
Measure, Clean, Repeat
When working with mammalian cells, they will stick to the FluidFM probe after an adhesion measurement. Thus the probe needs to be properly cleaned after each cell measured. Only once the probe is visibly clean a new cell measurement shoudl be started for best data quality.
Details on the cleaning procedure can be found in this article.