BIO Series / BIO Series Products / FluidFM QUANT

FluidFM QUANT SYSTEM

Deliver any drug into a cell, quantify the dose and study its effects at single cell level.

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Designed for pharmacologists and molecular biologists, the FluidFM QUANT system delivers any drug into living, adherent cells, while allowing a precise quantification of the injected dose, to ultimately study its effects at single-cell level.

Overcome the selectivity of the cell membrane permeability in the early drug development phase, by directly delivering the drug into the intracellular environment.

Cells natural heterogeneity (e.g. size) causes the injected volume to substantially differ, even under identical experimental conditions. The system allows to review each single injection and to precisely quantify the injected volume into each single cell. Establish a direct relation between the delivered drug concentration and its effects.

Robust and direct dose-response assays facilitate compliance with the most demanding regulations.


PRODUCT HIGHLIGHTS

Precisely select single cells based on their phenotype and deliver with the tiny FluidFM nanosyringe (aperture 600 nm) any kind of compounds in solution selectively into the nucleus or cytoplasm. The cell’s membrane instantaneously reseals when the nanosyringe is retracted, resulting in cell viability of over 95%.

When co-injected with a fluorescent marker, use this integrated software feature to precisely quantify how many femtoliters of the drug are added into the cell. The measurement after delivery, allows to know the true delivered volume, thus offering a robust method against natural cell-to-cell heterogeneity.

Benefit from simple preparation and extensive automation and data management combined with our user-friendly and intuitive software. Simply point and click on the cytoplasm or nucleus of the cells you want to target and click “OK”. It is as straightforward as that.

Only 1µl of solution is required to inject thousands of cells.

Fluorescence microscopy kit with three of the most frequently used filter sets (upgradable to 8), fully motorized, to distinguish relevant features of your cells and to follow the injection procedure in real-time by co-injection of fluorescent markers. 

Specifications:

  • Fluorescence light source: 130 W Hg light guide illumination

  • Motorized turret with 8 positions, built-in shutter, simple waterproof structurer; Single-band fluorescence cubes:
    (EF: Exciter filter, BS: Beam splitter, BF: Barrier filter)

    • Red: EF: 527 - 552 nm, BS: 565 nm, BF: 577 - 632 nm

    • Green: EF: 465 - 495 nm, BS: 505 nm, BF: 515 - 555 nm

    • Blue: EF: 360-370 nm, BS: 410 nm, BF: 420-460 nm

  • Fluorescence illuminator: Straight design with field iris diaphragm

pEGFP-UHRF1 and pmCherry-TRIM21 (2 plasmids) coinjected into CHO-K1 cells










Perform experiments within cell culture context, without perturbing it. Fully enclosing incubator with temperature control and a HEPA-Filter system, custom-designed around the QUANT system.

Specifications:

  • Black polycarbonate casing for optimal fluorescence imaging

  • Integrated, high precision temperature control system

  • Integrated airflow control system

  • Long-lasting, high Efficiency Particulate Air (HEPA) filter

  • 2 x 10W UV decontamination lamps with automatic life cycle monitoring & time control

  • 4 x white LED light source

  • Tailor-made doors for easy access to main QUANT system and microscope areas

  • Power consumption 620 W

Extra feature: CO2 controlled environment (coming soon)
When a CO2-independent medium is not a viable option, control of the CO2 concentration in the atmosphere is crucial. Then, it is advisable to equip the incubator with CO2-control capabilities.










Observe how the injected compounds affect the targeted cells in the long-term: Our software records the XY-coordinates of the injected cells and allows the user long-term observation of the modified cells with the desired settings (fluorescence filter, exposure time etc…). Observe any point of interest and follow how events unfold with multi-channel time-lapses.

Access to our database of proven injection protocols, see here.

The large XY-stage of the system can host standard well plates of the major brands, with up to 24 wells.

SOFTWARE

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Point-and-click

According to the high definition image of the cells on the screen, chose the cells of interest according to their phenotype. Select hundreds of cells under visual control, selectively target for the nucleus or cytoplasm.

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Easy walkthrough procedures

  • Follow the in-software suggested instructions to optimally deliver compounds into a cell

  • Apply recommended injection settings from our protocols database

  • Easily tailor injection parameters to your cells by changing few parameters (injection pressure, injection time, exerted force and approach speed)

  • Finally, step back and let the QUANT system autonomously deliver the drug

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Follow up your experiment

Instruct the system to take the needed images with your favorite settings, in few clicks. See all relevant data grouped by the coordinates of each cell.

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Volume Calculator – integrated feature

Simply select the images of the modified cells and follow the on-screen step-by-step instructions to obtain the real injected volume.

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Repeatability

Find the injected or chosen cells at any time, and even repeat injection into a same cell.

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CONSUMABLE - FluidFM nanosyringe

Developed for injection into single cells: The very sharp apex and the around 600 nm aperture at the pyramidal tip of the FluidFM nanosyringe enable gentle injection not compromising cell viability and allow to work with compounds with several molecular weights and densities. The FluidFM nanosyringe can be loaded with any liquid with viscosities from 1 to 10000 cP (water to honey).

  • Various cell lines – ideal for adherent mammalian cells like CHO, HeLa, HEK

  • Reliable –  inject into thousands of cells with the same nanosyringe

  • Easy handling – automatic consumable exchange and washing features

  • Gain ultimate insights – know the real shape of each nanosyringe in use by inspecting the SEM picture downloadable with the QR-Code printed on the blister

  • 1µl reservoir – reduce the amount of reagent: only 1µl of CRISPR-Cas solution is required to inject into thousands of cells

  • Microfabrication precision – the microchannel’s height serves as a precise volumetric reference for the correlation of the fluorescent signal with a volume

  • Specifications:

    • Aperture diameter: 600 nm

    • Tip & aperture shape: Pyramidal side aperture

    • Available spring constants: 2 N/m

    • Probe dimensions: Length 200 µm, Width 36 µm, Height 1 - 2.5 µm, Pyramid height 7 µm

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FluidFM nanosyringe aperture
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Specifications and real shape of the used FluidFM nanosyringe known by scanning the QR-code on the package

EMPOWER YOUR RESEARCH WITH FluidFM.


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