HANDLING COLLOIDAL PROBES.
Spherical colloids are the most suitable probes for local elasticity measurements on complex substrates. While colloidal probes are inherently difficult to produce and handle, FluidFM® technology overcomes these limitations in order to give you unparalleled flexibility for your most demanding research requirements.
60 µm polystyrene colloids are used to quickly asses cell adhesion. Courtesy of Dörig P,. ETH Zurich.
PROBES PER CANTILEVER
PROBE PER MINUTE
FluidFM probe amidst COOH beads, no bead is picked yet.
Reversible colloid attaching.
Imagine renewing your AFM colloidal probe in-situ without having to completely replace the entire probe. FluidFM technology makes opting for a completely fresh probe inherently easy. The simple, yet universal approach enabled by FluidFM technology allows to reversibly attach micro- and nanospheres to an atomic force cantilever in order to function as a colloidal probe.
Quantify long-term or irreversible interactions by using each colloidal probe only once. Fast, in-situ renewal of your probe is possible with FluidFM technology – at virtually no cost. Obtain solid statistics in short periods of time by measuring more data points than ever before. The versatility of FluidFM thereby allows you to use solid, liquid and gaseous colloids as required by your experiment.
Probe with a single BSA bead attached to the aperture.
Reversibly pick colloids from a surface or solution. Courtesy of Dörig P., ETH Zürich.
The colloids are seized and reversibly attached to the FluidFM probe by applying an underpressure to the microfluidic channel. Once measurement with the attached colloid concludes, it can be easily detached from the probe by application of a short overpressure pulse.
2013. P. Dörig, D. Ossola, A. M. Truong, M. Graf, F. Stauffer, J. Vörös & T. Zambelli.
Exchangeable colloidal AFM probes for the quantification of irreversible and long-term interactions.
Biophysical Journal, 105 (2), 463 – 472. doi:10.1016/j.bpj.2013.06.002
2015. B. R. Simona, L. Hirt, L. Demkó, T. Zambelli, J. Vörös, M. Ehrbar & V. Milleret.
Density gradients at hydrogel interfaces for enhanced cell penetration.
Biomater. Sci. doi:10.1039/C4BM00416G