a powerful tool for single cell biology.
FluidFM®BOT FOR INJECTION
SINGLE CELL EXPERIMENTS REINVENTED.
With the FluidFM BOT for Injection into single cells and the newly developed FluidFM nanosyringe, you can perform high-throughput injection of many compounds selectively into either cytoplasms or nuclei of adherent cell lines. This is achieved in a cell-context preserving and non-destructive manner.
Injection using the FluidFM technology is an incredible tool for biologists in cell line or drug development. Particularly, the FluidFM BOT for Injection has been designed specifically to address bottlenecks in the pipelines of drug development and genetic editing at the single cell level.
ANSWERS TO YOUR RESEARCH QUESTIONS.
The introduction of soluble compounds into selected, single adherent cells through FluidFM injection now allows researchers to answer questions such as:
How much of a drug is needed inside a cell for the desired effect to be activated (threshold concentration)?
What is the cell membrane permeability of pharmaceutical compounds?
How can I perform gene engineering on cells that are hard to transfect?
The FluidFM BOT is an unmatched tool to introduce a vast variety of materials of your choice into a cell, effortlessly.
From nanoparticles to proteins, CRISPR RNA, and plasmids, the FluidFM nanosyringe can be loaded with – and thus deliver – virtually any kind of liquid-based solution containing particles below 500 nm in diameter.
Efficiency of the microinjection using FluidFM technology has already been demonstrated in various kind of cell types such as human cervical cancer cells (HeLa), human embryonic kidney cells (HEK) or human induced pluripotent stem cells (hiPS).
O. Guillaume - Gentil, E. Potthoff, D. Ossola, P. Dörig, T. Zambelli & J. A. Vorholt. Force-controlled fluidic injection into single cell nuclei. (2013) Small, 9 (11), 1904 – 1907 . doi:10.1002/smll.201202276
Meister, M. Gabi, P. Behr, P. Studer, J. Vörös, P. Niedermann, J. Bitterli, J. Polesel-Maris, M. Liley, H. Heinzelmann & T. Zambelli. FluidFM: Combining atomic force microscopy and nanofluidics in a universial liquid delivery system for single cell applications and beyond. (2009) Nano Letters, 9 (6), 2501 – 2507. doi:10.1021/nl901384x
injected volume estimation.
Working with fluorescent compounds, the microfabricated FluidFM nanosyringe serves as a precise reference volume for fluorescence intensity.
Due to manufacturing precision, the constant dimension of the FluidFM nanosyringe allows an accurate estimation of the injected volume for each cell, by measuring the cells fluorescence intensity after injection in comparison with that of the FluidFM nanosyringe.
To facilitate the calculation of the injected volume, we provide an easy to use ImageJ macro. This volume information is key for many experiments, for example when determining a dose-response relationship.
FluidFM EMPOWERS CRISPR GENE EDITING.
Perform fast injection of CRISPR-Cas complexes, even in cell lines difficult to modify with conventional CRISPR delivery methods.
Using the FluidFM BOT for Injection to deliver your CRISPR-Cas complex, you can selectively choose the cell you want to edit within a cell culture. This is performed without affecting the neighboring cells.
Injection time for the complex is only 2 seconds per cell with a gentle 75 mbar pressure corresponding to approximately 30 femtoliters of injected volume.
With FluidFM injection, deliver your CRISPR-Cas complex directly to where it is required: the nucleus.
TOWARDS FULL AUTOMATION.
A high definition image of the cells appears on the screen. Simply click with the mouse on the cytoplasm or the nucleus of the targeted cells, and press “OK”. It is as simple as that.
Simple preparation, extensive automation and our intuitive FluidFM ARYA operator software allows more or less any user to inject more than a hundred cells per hour. For the injection of Lucifer Yellow dye into HeLa cells, average rates of 100-300 cells per hour can easily be reached.
The software-empowered injection specific workflow allows all users to be in full control of their experimental parameters and eliminates worries on the operation of the instrument.
Due to the fully programmable FluidFM nanosyringe exchange and washing features, all experiments are executed in a clean and precise fashion reducing contamination risks and human error to a minimum.
The FluidFM BOT system boosts productivity by minimizing laborious sample swapping and manual intervention during measurements.
FluidFM BOT FOR INJECTION.
A high level of automation coupled with intuitive instrument operation have proven to be particularly important for injection into single cells.
The FluidFM BOT system takes FluidFM injection to the next level, thanks to the state-of-the-art long-range, high-precision XY-stage, extensive automation and microscope integration.
The accompanying FluidFM ARYA operator software has been designed with usability and integrability in mind: from system preparation, through experiment execution to data management.
The FluidFM BOT for Injection is a complete standalone system which includes elements such as a new state-of-the- art microfluidics controller, an inverted microscope, a made-to-measure incubator, an anti-vibration table and a set of FluidFM probes, including the FluidFM nanosyringe.
FluidFM unites the best features of microfluidics and force microscopy by introducing microscopic channels into force sensitive FluidFM probes, such as the FluidFM nanosyringe.
These microfabricated FluidFM probes are at the heart of the patented FluidFM technology. Through the microfluidic channel inside the probes, soluble molecules can be dispensed through a sub-micrometer aperture at the tip.
These apertures can be as small as 300 nm, enabling femtoliter volumes to be handled by accurately controlling positive or negative pressures with 0.2 mbar precision through the probe.
FluidFM technology elevates the application scope of traditional glass micropipettes to new levels.