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Working with the World's Smallest Syringe.
Injection into single cells
FluidFM INJECTION INTO SINGLE CELLS.
With FluidFM, the world’s smallest syringe, single cell injection is easy. Change a single cell within a culture and study the cascade effect on its surroundings.
Single cell injection enables targeted introduction of foreign materials into a specific cell. Stimulating selected cells within a colony with your desired drugs or gene vectors provides new insights for life science, biology and medicine.
Choose FluidFM microinjection as a strong addition to your experimental portfolio.
PRECISE
IN CYTOPLASM OR NUCLEUS
FAST
100+ CELLS / HOUR
GENTLE
95%+ CELL VIABILITY
MEASURABLE
FEMTOLITER INJECTED VOLUMES
Fast And Reliable.
During an injection with FluidFM, the inherent force feedback protects both the cell and the FluidFM probe. Thus, all injections are successful and cells survive the operation unharmed.
A single probe can inject hundreds of cells per hour in an automated process. Simply click on a cell and watch the injection under the microscope. This provides high throughput and makes injection both easy and repeatable.
Simplified Working Methods.
Highly automated workflows and our intuitive monitoring software support users in every step of their experiment.
The user selects a cell by simply clicking it, based on fluorescent, morphological, or mechanical markers. While monitoring the force, the FluidFM probe approaches the cell and penetrates its membrane. The substance of choice is then injected via a short pressure pulse, and the FluidFM probe retracts again.
SELECTED PUBLICATIONS
2013
O. Guillaume - Gentil, E. Potthoff, D. Ossola, P. Dörig, T. Zambelli & J. A. Vorholt.
Force-controlled fluidic injection into single cell nuclei.
Small, 9 (11), 1904
– 1907
. doi:10.1002/smll.201202276
2009
Meister, M. Gabi, P. Behr, P. Studer, J. Vörös, P. Niedermann, J. Bitterli, J. Polesel-Maris, M. Liley, H. Heinzelmann & T. Zambelli.
FluidFM: Combining atomic force microscopy and nanofluidics in a universial liquid delivery system for single cell applications and beyond.
Nano Letters, 9 (6), 2501 – 2507. doi:10.1021/nl901384x