Silicon Nitride (SiN)
200 x 36 x 1.7 µm (LxWxH)
Spring Constant (nominal):
Pyramidal side aperture
600 nm nominal
10 x 10 x 7 µm
Cr/Au (40 nm)
What are the typical flows and volumes involved when working with FluidFM probes?
The flow of water through a FluidFM cantilever is typically in the range of fl/s to pL/s. This is 1000x below even the best flow sensors, which have detection limits of around 1 nL/s. Accordingly the dispensed volumes start at single fL for applications such as spotting in air or injecting cells.
How long can I use the same FluidFM probe?
The lifetime of a FluidFM probe depends greatly on the experimental conditions, as well as on the applied filling, cleaning and storage protocols. Thus, a FluidFM probe’s lifetime varies between one experiment (a few hours) and one month. Please contact us for advice on the optimal probe protocols for your experiment.
Selected Nanosyringe applications
Perform FluidFM nano-injection with a vast variety of compounds selectively into either cytoplasms or nuclei of adherent cells, in a cell-context preserving, non-destructive (cell viability >95%), measurable (fl volumes) and fast (inject hundreds of cells/hour) manner.
Extract the content of individual cells directly in their native environment while preserving the cellular context and without affecting cell viability. The procedure is non-destructive and therefore makes it possible to perform cell biopsies from the same single cell over different time points.