FluidFM nanosyringe
FluidFM nanosyringe
Specifications
Material: | Silicon Nitride (SiN) |
Cantilever Dimensions: Channel Height: | 200 x 36 x 1.7 µm (LxWxH) 1500 nm |
Spring Constant (nominal): | 2.2 N/m |
| Opening Type: | Pyramidal side aperture |
Opening Dimension: Pyramid Dimensions: | 600 nm nominal
10 x 10 x 7 µm |
Reflective Coating: | Cr/Au (40 nm) |
FAQ
What are the typical flows and volumes involved when working with FluidFM probes?
The flow of water through a FluidFM cantilever is typically in the range of fl/s to pL/s. This is 1000x below even the best flow sensors, which have detection limits of around 1 nL/s. Accordingly the dispensed volumes start at single fL for applications such as spotting in air or injecting cells.
How long can I use the same FluidFM probe?
The lifetime of a FluidFM probe depends greatly on the experimental conditions, as well as on the applied filling, cleaning and storage protocols. Thus, a FluidFM probe’s lifetime varies between one experiment (a few hours) and one month. Please contact us for advice on the optimal probe protocols for your experiment.
Selected Nanosyringe applications
Nano-injection
Perform FluidFM nano-injection with a vast variety of compounds selectively into either cytoplasms or nuclei of adherent cells, in a cell-context preserving, non-destructive (cell viability >95%), measurable (fl volumes) and fast (inject hundreds of cells/hour) manner.
nano-extraction
Extract the content of individual cells directly in their native environment while preserving the cellular context and without affecting cell viability. The procedure is non-destructive and therefore makes it possible to perform cell biopsies from the same single cell over different time points.
