1. Slides: FluidFM nano-injection - FluidFM BOT Webinar

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9. A WORLD OF APPLICATIONS .

13. NANO - INJECTION .

14. 14

3. FluidFM . WHAT IS IT?

5. A CLOSE LOOK. FluidFM PROBES. FluidFM Chip Probe = Consumable Zoom in

23. ISOLATION . NANO - EXTRACTION .

24. ISOLATION OF SINGLE CELLS .

25. 28

32.

1. FluidFM BOT WEBINAR. SINGLE CELL EXPERIMENTS. Nano - injection , Cell isolation, Nano - extraction. March 2019. Dr. Paul Monnier, Dr. Pablo Dörig

28. 31

29. S WISS MADE TECHNOLOGY .

31. UPCOMING WEBINAR.

33. LIVE DEMO. NANO - INJECTION.

34. GO BEYOND.

4. PATENTED KNOW - HOW. FluidFM. Fluidic Force Microscopy. FluidFM: Meister & Gabi et al., Nano Lett. 9, 2009

12. 12 FULLY INTEGRATED SYSTEM SOLUTION. F luid FM ® B O T.

2. 60 MINUTES OF FluidFM . What is FluidFM? Single cell applications Live Demo 2 THE WEBINAR TODAY.

6. 300 – 800 nm hole NANOSYRINGE A CLOSE LOOK II. FluidFM PROBES.

11. 11 NANO - EXTRACTION FROM SINGLE CELLS NANO - INJECTION INTO SINGLE CELLS ISOLATION OF SINGLE CELLS F luid FM OVERVIEW. SINGLE CELL APPLICATIONS.

15. 300 – 800 nm hole NANOSYRINGE NANO - INJECTION. FluidFM NANOSYRINGE.

10. NANO - EXTRACTION FROM SINGLE CELLS NANO - INJECTION INTO SINGLE CELLS ISOLATION OF SINGLE CELLS SPOTTING NANOLITHOGRAPHY ADHESION OF SINGLE CELLS ADHESION OF SINGLE BACTERIA COLLOIDAL SPECTROSCOPY SICM PATCH CLAMP NANOBALANCE 10 3D PRINTING Application videos : https://www.cytosurge.com/slides Find all FluidFM publications: https://www.cytosurge.com/page/publications F luid FM TECHNOLOGY. A WORLD OF APPLICATIONS.

21. F luid FM ® NANO - INJECTION. 23 VOLUME CALCULATION .

20. F luid FM ® NANO - INJECTION. 22 CELL VIABILITY UP TO 95% Injection of Lucifer yellow in CHO cells Propidium iodide staining 10 min after injection

18. F luid FM ® NANO - INJECTION. 19 INJECTION INTO ADHERENT CELL LINES . HepG2 Mouse primary hepatocytes HEK Pig neurons C2C12 MEFs hESCs mESCs

19. F luid FM ® NANO - INJECTION. 21 INJECTING SOLUBLE COMPOUNDS. Lucifer Yellow UHRF1 proteins Antibodies CRISPR/Cas9 RNP complexes Plasmids Small RNAs

22. F luid FM ® NANO - INJECTION. 24 VOLUME CALCULATION (2). HeLa cells CHO cells Mouse primary hepatocyte HepG2 cells

8. FluidFM . MORE THAN A JUST ONE TOOL. 8 MICROPIPETTE GRAB & MANIPULATE MICRO OBJECTS NANOSYRINGE INJECT AND EXTRACT NNOPIPETTE LITHOGRAPHY AND MUCH MORE

26. NANO - EXTRACTION . SINGLE CELL NANO - EXTRACTION. A fluorescent myoblast is contacted by FluidFM ® . Orane Guillaume - Gentil, Analytical Chemistry - 10.1021/acs.analchem.7b00367

16. F luid FM ® NANO - INJECTION. 16 FORCE FEEDBACK ALLOWS AUTOMATION. HeLa CELLS EXPRESSED GFP GFP PLASMID 50 μ m Guillaume - Gentil et al. (2012) , Small 9

27. F luid FM ® B O T. 30 NANO - EXTRACTION. O. Guillaume - Gentil et al., Cell 2016, 166: 506 - 516 Anti - fouling coating Cell extract Oil Channel filled with oil

17. F luid FM ® NANO - INJECTION.  >300 cells injected per hour  10 minutes preparation time  >90% succesfull injection 18 NANO - INJECTION WITH THE FluidFM B O T.

7. FluidFM ® PROBES PORTFOLIO THE HEART OF FluidFM. FluidFM ® micropipette 0.3 – 4N/m, aperture sizes: 2, 4, 8 μm Isolation & adhesion of single cells , colloidal spectroscopy, local dispensing FluidFM ® nanopipette 0 .6 – 2N/m, aperture size: 300 nm N ano - printing, manipulation of sub μm particles, adhesion of single bacteria FluidFM ® nanosyringe 2 N/m, aperture size: 300 - 800 nm Nano - injection into & nano - extraction from single cells FluidFM ® prototyping probe A perture can be customized with Focused Ion Beam (FIB) A pplication depending on the customization 300 nm to 8 μm PRECISE CUSTOM SHAPES STANDARD OPENING DIAMETERS BATCH FABRICATION DOWN TO 30 nm POSSIBLE WITH FIB

30.  Founded 2009 in Zurich, Switzerland  Dr. Pascal Behr (CEO)  Dr. Michael Gabi (CTO)  ETH Zurich Spin - off  20+ employees in 2019  Prize winning company THE COMPANY BEHIND FLUIDFM. CYTOSURGE AG. Ø 7 + IMPACT FACTOR 60+ PUBLICATIONS HIGH IMPACT JOURNALS 60+ SYSTEMS WORLDWIDE 2013 FIRST SALE 35

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