2.1 Video: Cell isolation with the FluidFM BOT - Detailed Experimental Procedure

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Please watch the video on YouTube for jumping directly to specific chapters.

VIDEO CONTENT:
1. Manual preparation 0:26
2. Software preparation workflow 3:31
2.1 Pacram coating 05:55
3. Placing a well plate into BOT for isolating cells 10:13
4. Rinsing FluidFM probe with PBS 12:43
5. Setting parameters 13:36
6.Showering cells with trypsin 14:36
7. Cell isolation
7.1. Cell isolation 18:43
7.2. Cell isolation 23:05
7.3. Cell isolation 26:37
7.4. Cell isolation 30:24
7.5. Cell isolation 32:04
7.6. Cell isolation 33:36
7.7. Cell isolation 35:34
7.8. Cell isolation 37:04
7.9. Cell isolation 38:28
7.10. Cell isolation 39:59
7.11. Cell isolation 41:38
7.12. Cell isolation 44:17
8. After cell isolation: next steps 46:23

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Please watch the video on YouTube for jumping directly to specific chapters.

VIDEO CONTENT:

1. Manual preparation 0:26

2. Software preparation workflow 3:31

- Pacram coating 05:55

3. Placing a well plate into BOT for isolating cells 10:13

4. Rinsing FluidFM probe with PBS 12:43

5. Setting parameters 13:36

6.Showering cells with trypsin 14:36

7. Cell isolation

1. Cell isolation 18:43

2. Cell isolation 23:05

3. Cell isolation 26:37

4. Cell isolation 30:24

5. Cell isolation 32:04

6. Cell isolation 33:36

7. Cell isolation 35:34

8. Cell isolation 37:04

9. Cell isolation 38:28

10. Cell isolation 39:59

11. Cell isolation 41:38

12. Cell isolation 44:17

8. After cell isolation: next steps 46:23

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