FluidFM ADD-ON for your Nanosurf AFM system | Factsheet

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Empower your Nanosurf AFM beyond imaging. The FluidFM ADD-ON from Cytosurge opens the door to a wide range of new applications. From single cell biology to material sciences – harness the possibilities provided by FluidFM using the AFM you already own.

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1. Subject to change without notice. Revision: 1.0 Copyright © 2019 Cytosurge AG, Switzerland 100% FluidFM ® APPLICATIONS 10 0% AFM CAPABILITIES pN FORCE CONTROL sub-nm XY Z-RESOLUTION POWERFUL UPGRADE SOLUTION. F luid FM ® ADD-ON FOR YOUR NANOSURF AFM SYSTEM Empower your Nanosurf AFM beyond imaging. The FluidFM ADD-ON from Cytosurge opens the door to a wide range of new applications. From single cell biology to material sciences – harness the possibilities provided by FluidFM using the AFM you already own. Applications ranging from single cell isolation to colloidal spectroscopy measurements become readily possible with this powerful upgrade solution. BioAFM users have now, for example, access to single cell gene editing, much faster mechanical CLIP IT ON. Close-up of the Nanosurf FlexAFM scan head with a mounted FluidFM probe. POWERFUL UPGRADE SOLUTION. A FluidFM ADD-ON installed on a Nanosurf FlexAFM. 1/4 PROBE HOLDER PNEUMATIC CONNECTOR FluidFM ® PROBE:

2. Subject to change without notice. Revision: 1.0 Copyright © 2019 Cytosurge AG, Switzerland F luid FM ® ADD-ON FOR YOUR NANOSURF AFM SYSTEM measurements like single cell force spectroscopy (SCFS), and direct printing of sub-micron feature patterns. FluidFM also enables the easy manipulation and measurement of micron-sized colloids, mammalian cells, microorganisms such as yeast, algae, bacteria, or even single virus particles. UNLEASH YOUR AFM. With the three distinct FluidFM probe families you get access to unique and high impact applications. More information on the diverse applications: www.cytosurge.com/page/fluidfm-addon F luid FM ® ADD-ON. TAILORED TO YOUR NEEDS. The FluidFM ® ADD-ON is compatible with all Nanosurf FlexAFM and CoreAFM models. All the capabilities of the optical as well as the atomic force microscope are fully available during FluidFM operations. To offer you the best possible solution for your requirements, we have created a selection of bespoke packages. The Silver package is the ideal choice for researchers looking for an economical start in the world of FluidFM. The Silver package offers 12 months warranty and includes a FluidFM probe Starter kit containing 3 probes. The Gold package is the most popular choice, maximizing the application range through software integration of the FluidFM system into the Nanosurf software. The Gold package ensures a smooth journey in the world of FluidFM and includes a FluidFM probe Explorer kit containing a selection of 10 micropipettes and nanopipettes of various stiffnesses and apertures. You also benefit from 24 months warranty on the system. The Platinum package is the best choice if you want to experience the full power of the premium FluidFM ADD-ON solution. With a comprehensive integration into the Nanosurf software, it combines the full FluidFM application range with highest ease of use, leading also to highest possible throughputs. With the Platinum package, you have 36 months warranty and you can take advantage of the comprehensive FluidFM probe Discovery kit with 20 probes. See the detailed information about the packages on the next page. F lex FPM WITH ARYA. The FlexFPM features our easy and intuitive in-house ARYA software designed specifically for all popular FluidFM applications. Nanosurf’s C3000 software is still fully functional and runs in parallel to ARYA. FluidFM micropipette FluidFM nanosyringe FluidFM nanopipette 2, 4, 8 μm aperture 600 nm aperture 300 nm aperture A dhesion of single cells C olloidal spectroscopy C ell isolation N ano-injection into single cells N ano-extraction from single cells Spotting Adhesion of single bacteria Nano- printing 2/4 The mentioned applications are enabled by the unique and patented FluidFM probes, tailored to the geometrical and physical requirements of the AFM. Each probe type with its specific tip shape, aperture size and mechanical properties can be used for several applications.

4. F luid FM ® ADD-ON FOR YOUR NANOSURF AFM SYSTEM 4/4 The white cells show the product codes and the amount of this type contained in the kit. The white cells show the product codes and the amount of this type contained in the kit. The white cells show the product codes and the amount of this type contained in the kit. Stiffness in N/m Kit Code: 1979 0.3 0.6 2 4 Apertrue in μm Nanopipette 0.3 1 x 1343 Nanosyringe 0.8 Micro- pipettes 2 1 x 1205 4 8 1 x 1211 Stiffness in N/m Kit Code: 2006 0.3 0.6 2 4 Apertrue in μm Nanopipette 0.3 1 x 1457 2 x 1343 Nanosyringe 0.8 Micro- pipettes 2 1 x 1206 2 x 1205 4 1 x 1209 1 x 1208 8 1 x 1212 1 x 1211 Stiffness in N/m Kit Code: 2010 0.3 0.6 2 4 Apertrue in μm Prototyping n.a. 1 x 2631 1 x 2639 Nanopipette 0.3 2 x 1457 2 x 1343 Nanosyringe 0.8 4 x 2072 Micro- pipettes 2 2 x 1206 2 x 1205 4 1 x 1209 2 x 1208 8 1 x 1212 2 x 1211 FluidFM Starter kit FluidFM Explorer kit FluidFM Discovery kit Subject to change without notice. Revision: 1.0 Copyright © 2019 Cytosurge AG, Switzerland CYTOSURGE AG, SÄGEREISTRASSE 25, 8152 GLATTBRUGG, CH PHONE +41 43 544 87 00, WWW.CYTOSURGE.COM

3. Subject to change without notice. Revision: 1.0 Copyright © 2019 Cytosurge AG, Switzerland F luid FM ® ADD-ON FOR YOUR NANOSURF AFM SYSTEM 3/4 FluidFM ® ADD-ON Packages SILVER GOLD PLATINUM F lex FPM 1) Probes kit Predefined selection of different probes of various stiffnesses and apertures. 3 probes FluidFM Starter kit 10 probes FluidFM Explorer kit 20 probes FluidFM Discovery kit 20 probes FluidFM Discovery kit Support Provided by Nanosurf. ü ü ü ü Warranty (in months) 12 24 36 36 FluidFM MFCS Microfluidics control unit + pump unit + tubing. To aspirate or release liquids through the FluidFM probe. V1 V2 2) V2 2) V2 2) FluidFM CORA operator software Including free software updates. ü ü ü ü AFM specific accessories Customized solution for your AFM: Pneumatic connections, adapters and a tailor-made FluidFM probe holder assuring safe and reliable mounting of FluidFM probes. ü ü ü ü Barcode reader module Integrated into software. T o access all relevant probe characteristics, most importantly a unique SEM image of every probe. ü ü ü ü FluidFM liquid trap Reduces the risk of liquid damage. ü ü ü API access to FluidFM microfluidics control system ü ü ü FluidFM manual pressure kit For additional under- and overpressure. ü ü ü Standard software integration Control FluidFM within the AFM software. ü FluidFM camera module A dedicated FluidFM fluorescence camera with a digital filling assistant for reliable detection of probe filling. ü ü Premium software integration Advanced FluidFM workflows and tools within the AFM software. ü ARYA software + PC and touchscreen monitor Point & click FluidFM integration. Easy and powerful. ü 1) With ARYA software - fully integrated FluidFM experience. 2) Compared to its predecessor, the FluidFM MFCS V2 features a new integrated leak detection guaranteeing that every probe is tightly gripped before an experiment.

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