FluidFM Products Overview - Flyer

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FluidFM - Nano Precision, Diverse Applications.

Please download the flyer to gain an overview of our FluidFM products:
FluidFM ADD-ON, FluidFM BOT, FluidFM µ3Dprinter

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5. Please contact us with your specific request or application idea. We and our distributors will be delighted to be able to support you and help make your vision come true. CYTOSURGE AG, SÄGEREISTRASSE 25, 8152 GLATTBRUGG, SWITZERLAND PHONE +41 43 544 87 00, FAX +41 43 544 87 09, WWW.CYTOSURGE.COM Copyright © 2018 Cytosurge AG, Switzerland CONTAC T US. NANO PRECISION. DIVERSE APPLICATIONS. CUTTING EDGE & UNIQUE. FluidFM technology unites the best features of microfluidics and force microscopy by introducing a microscopic channel into force sensitive probes. The result is the FluidFM probe, the heart of our patented FluidFM technology. Through the microfluidic channel inside the FluidFM probe, soluble molecules and nanoparticles can be dispensed or aspirated through a sub-micrometer aperture at the tip. These apertures can be as small as 300 nm enabling the handling of femtoliter volumes by accurately controlling positive and negative pressures with sub-mBar precision. The force-sensing capability of FluidFM probes provides a reliable feedback about physical interactions. This enables distinction between gentle contact with cell membranes and their perforation. It can equally provide active feedback signals for real-time process control during printing. Force-controlled manipulation of microscopic objects is now possible. HIGH QUALITY MICROFABRICATION PRECISE FEMTOLITER LIQUID VOLUMES VERSATILE 12+ APPLICATIONS TECHNOLOGY 200 nm INJECTION INTO SINGLE CELLS NANOLITHOGRAPHY SPOTTING EXTRACTION FROM SINGLE CELLS ADHESION OF SINGLE BACTERIA COLLOIDAL SPECTROSCOPY ADHESION OF SINGLE CELLS NANOBALANCE 3D PRINTING ELECTRO- CHEMISTRY ISOLATION OF SINGLE CELLS A WORLD OF APPLICATIONS SURFACE MANIPULATION GO BEYOND.

6. Please contact us with your specific request or application idea. We and our distributors will be delighted to be able to support you and help make your vision come true. CYTOSURGE AG, SÄGEREISTRASSE 25, 8152 GLATTBRUGG, SWITZERLAND PHONE +41 43 544 87 00, FAX +41 43 544 87 09, WWW.CYTOSURGE.COM Copyright © 2018 Cytosurge AG, Switzerland CONTAC T US. NANO PRECISION. DIVERSE APPLICATIONS. CUTTING EDGE & UNIQUE. FluidFM technology unites the best features of microfluidics and force microscopy by introducing a microscopic channel into force sensitive probes. The result is the FluidFM probe, the heart of our patented FluidFM technology. Through the microfluidic channel inside the FluidFM probe, soluble molecules and nanoparticles can be dispensed or aspirated through a sub-micrometer aperture at the tip. These apertures can be as small as 300 nm enabling the handling of femtoliter volumes by accurately controlling positive and negative pressures with sub-mBar precision. The force-sensing capability of FluidFM probes provides a reliable feedback about physical interactions. This enables distinction between gentle contact with cell membranes and their perforation. It can equally provide active feedback signals for real-time process control during printing. Force-controlled manipulation of microscopic objects is now possible. HIGH QUALITY MICROFABRICATION PRECISE FEMTOLITER LIQUID VOLUMES VERSATILE 12+ APPLICATIONS TECHNOLOGY 200 nm INJECTION INTO SINGLE CELLS NANOLITHOGRAPHY SPOTTING EXTRACTION FROM SINGLE CELLS ADHESION OF SINGLE BACTERIA COLLOIDAL SPECTROSCOPY ADHESION OF SINGLE CELLS NANOBALANCE 3D PRINTING ELECTRO- CHEMISTRY ISOLATION OF SINGLE CELLS A WORLD OF APPLICATIONS SURFACE MANIPULATION GO BEYOND.

1. Please contact us with your specific request or application idea. We and our distributors will be delighted to be able to support you and help make your vision come true. CYTOSURGE AG, SÄGEREISTRASSE 25, 8152 GLATTBRUGG, SWITZERLAND PHONE +41 43 544 87 00, FAX +41 43 544 87 09, WWW.CYTOSURGE.COM Copyright © 2018 Cytosurge AG, Switzerland CONTAC T US. NANO PRECISION. DIVERSE APPLICATIONS. CUTTING EDGE & UNIQUE. FluidFM technology unites the best features of microfluidics and force microscopy by introducing a microscopic channel into force sensitive probes. The result is the FluidFM probe, the heart of our patented FluidFM technology. Through the microfluidic channel inside the FluidFM probe, soluble molecules and nanoparticles can be dispensed or aspirated through a sub-micrometer aperture at the tip. These apertures can be as small as 300 nm enabling the handling of femtoliter volumes by accurately controlling positive and negative pressures with sub-mBar precision. The force-sensing capability of FluidFM probes provides a reliable feedback about physical interactions. This enables distinction between gentle contact with cell membranes and their perforation. It can equally provide active feedback signals for real-time process control during printing. Force-controlled manipulation of microscopic objects is now possible. HIGH QUALITY MICROFABRICATION PRECISE FEMTOLITER LIQUID VOLUMES VERSATILE 12+ APPLICATIONS TECHNOLOGY 200 nm INJECTION INTO SINGLE CELLS NANOLITHOGRAPHY SPOTTING EXTRACTION FROM SINGLE CELLS ADHESION OF SINGLE BACTERIA COLLOIDAL SPECTROSCOPY ADHESION OF SINGLE CELLS NANOBALANCE 3D PRINTING ELECTRO- CHEMISTRY ISOLATION OF SINGLE CELLS A WORLD OF APPLICATIONS SURFACE MANIPULATION GO BEYOND.

2. POWERFUL UPGRADE SOLUTION FOR YOUR AFM. The FluidFM ADD-ON extends the application scope of classical atomic force microscopes (AFMs) way beyond imaging and force spectroscopy. Take advantage of more than 12 applications, now accessible thanks to the FluidFM ADD-ON. The FluidFM ADD-ON is tailored to many AFM scan heads and brings you the highest fl exibility by enabling all FluidFM applications – from single cell adhesion, through injection into single cells and colloidal studies, to nanolithography and beyond. You can even go a step further and leverage FluidFM using the custom- developed solutions of our Premium Partners enabling a seamless user experience, or even develop your own applications. With the FluidFM ADD-ON, your AFM has never been more powerful. INJECTION INTO SINGLE CELLS REINVENTED. 3D METAL PRINTING AT MICROMETER SCALE. With the FluidFM BOT , you can perform nano injection of a vast variety of materials of your choice selectively into either cytoplasms or nuclei of adherent cell s . Inject into hundreds of cells per hour in a cell-context preserving , non-destructive and measurable manner. From nanoparticles to proteins, CRISPR RNA and plasmids, you can inject virtually any kind of liquid-based solutions containing particles below 500 nm in diameter. Success of microinjection using the FluidFM BOT has already been demonstrated in various kinds of cell types such as human cervical cancer cells (HeLa), human embryonic kidney cells (HEK) or human induced pluripotent stem cells (hiPS). Th e FluidFM BOT is a revolutionary tool for gene engineering, epigenetics, pharmaceutical drug development, toxicology, stem and cancer cell research, cell line development and many other cell and bio science research areas. As a stand-alone system and with a high level of automation and intuitive instrument operation, the FluidFM BOT is a remarkably powerful tool for s ing le cell biology. PRECISE CYTOPLASM OR NUCLEUS FAST 100+ CELLS / HOUR GENTLE 95%+ CELL VIABILITY The FluidFM μ3Dprinter offers truly new capabilities in manufacturing complex microscopic and submicroscopic metal structures. Micrometer- accurate 3D printing revolutionizes micro-manufacturing by combining additive manufacturing with traditional microfabrication methods. The FluidFM μ3Dprinter is a stand-alone system for printing metal objects at micrometer scale. The printed structure size is 1 μm 3 to 1’000’000 μm 3 , a range virtually inaccessible by other techniques. Practically any design can be printed by the system, including overhanging structures with 90 degree angles, without the need for support structures or post-processing steps. The unique technology of the FluidFM μ3Dprinter is capable of being industrially scalable. Additionally, the FluidFM μ3Dprinter also allows the dispensing of various liquids and nanoparticles. These capabilities lead to new research opportunities in fields such as microelectronics, semiconductors, surface modification, material science and many more. The FluidFM μ3Dprinter is the ideal system for metal micro-object manufacturing and for dispensing liquids as well as nanoparticles. BOT μ 3D printer MEASURABLE FEMTOLITER INJECTED VOLUMES PRECISE MICROMETER RESOLUTION REAL - T IME PROCESS C ONTROL VERSATILE VARIOUS MATERIALS FAST up to 100 μm/s ADD-ON 2 μm FluidFM ADD-ON. Empower your AFM. SPOTTING. One of many applications made possible by the FluidFM ADD-ON. FluidFM BOT. Stand-alone system for microinjection (incubator not shown). GENTLE MICROINJECTION. Repeatedly inject into the same living cell. FluidFM μ3Dprinter. Stand-alone system for printing metal objects at micrometer scale. PRINTED OBJECT. Printed in one single step and without support structures. 12+ APPLICATIONS 100% AFM CAPABILITIES MAINTAINED TAILORED AFM SOLUTION CUSTOMIZED SOLUTIONS POSSIBLE

3. POWERFUL UPGRADE SOLUTION FOR YOUR AFM. The FluidFM ADD-ON extends the application scope of classical atomic force microscopes (AFMs) way beyond imaging and force spectroscopy. Take advantage of more than 12 applications, now accessible thanks to the FluidFM ADD-ON. The FluidFM ADD-ON is tailored to many AFM scan heads and brings you the highest fl exibility by enabling all FluidFM applications – from single cell adhesion, through injection into single cells and colloidal studies, to nanolithography and beyond. You can even go a step further and leverage FluidFM using the custom- developed solutions of our Premium Partners enabling a seamless user experience, or even develop your own applications. With the FluidFM ADD-ON, your AFM has never been more powerful. INJECTION INTO SINGLE CELLS REINVENTED. 3D METAL PRINTING AT MICROMETER SCALE. With the FluidFM BOT , you can perform nano injection of a vast variety of materials of your choice selectively into either cytoplasms or nuclei of adherent cell s . Inject into hundreds of cells per hour in a cell-context preserving , non-destructive and measurable manner. From nanoparticles to proteins, CRISPR RNA and plasmids, you can inject virtually any kind of liquid-based solutions containing particles below 500 nm in diameter. Success of microinjection using the FluidFM BOT has already been demonstrated in various kinds of cell types such as human cervical cancer cells (HeLa), human embryonic kidney cells (HEK) or human induced pluripotent stem cells (hiPS). Th e FluidFM BOT is a revolutionary tool for gene engineering, epigenetics, pharmaceutical drug development, toxicology, stem and cancer cell research, cell line development and many other cell and bio science research areas. As a stand-alone system and with a high level of automation and intuitive instrument operation, the FluidFM BOT is a remarkably powerful tool for s ing le cell biology. PRECISE CYTOPLASM OR NUCLEUS FAST 100+ CELLS / HOUR GENTLE 95%+ CELL VIABILITY The FluidFM μ3Dprinter offers truly new capabilities in manufacturing complex microscopic and submicroscopic metal structures. Micrometer- accurate 3D printing revolutionizes micro-manufacturing by combining additive manufacturing with traditional microfabrication methods. The FluidFM μ3Dprinter is a stand-alone system for printing metal objects at micrometer scale. The printed structure size is 1 μm 3 to 1’000’000 μm 3 , a range virtually inaccessible by other techniques. Practically any design can be printed by the system, including overhanging structures with 90 degree angles, without the need for support structures or post-processing steps. The unique technology of the FluidFM μ3Dprinter is capable of being industrially scalable. Additionally, the FluidFM μ3Dprinter also allows the dispensing of various liquids and nanoparticles. These capabilities lead to new research opportunities in fields such as microelectronics, semiconductors, surface modification, material science and many more. The FluidFM μ3Dprinter is the ideal system for metal micro-object manufacturing and for dispensing liquids as well as nanoparticles. BOT μ 3D printer MEASURABLE FEMTOLITER INJECTED VOLUMES PRECISE MICROMETER RESOLUTION REAL - T IME PROCESS C ONTROL VERSATILE VARIOUS MATERIALS FAST up to 100 μm/s ADD-ON 2 μm FluidFM ADD-ON. Empower your AFM. SPOTTING. One of many applications made possible by the FluidFM ADD-ON. FluidFM BOT. Stand-alone system for microinjection (incubator not shown). GENTLE MICROINJECTION. Repeatedly inject into the same living cell. FluidFM μ3Dprinter. Stand-alone system for printing metal objects at micrometer scale. PRINTED OBJECT. Printed in one single step and without support structures. 12+ APPLICATIONS 100% AFM CAPABILITIES MAINTAINED TAILORED AFM SOLUTION CUSTOMIZED SOLUTIONS POSSIBLE

4. POWERFUL UPGRADE SOLUTION FOR YOUR AFM. The FluidFM ADD-ON extends the application scope of classical atomic force microscopes (AFMs) way beyond imaging and force spectroscopy. Take advantage of more than 12 applications, now accessible thanks to the FluidFM ADD-ON. The FluidFM ADD-ON is tailored to many AFM scan heads and brings you the highest fl exibility by enabling all FluidFM applications – from single cell adhesion, through injection into single cells and colloidal studies, to nanolithography and beyond. You can even go a step further and leverage FluidFM using the custom- developed solutions of our Premium Partners enabling a seamless user experience, or even develop your own applications. With the FluidFM ADD-ON, your AFM has never been more powerful. INJECTION INTO SINGLE CELLS REINVENTED. 3D METAL PRINTING AT MICROMETER SCALE. With the FluidFM BOT , you can perform nano injection of a vast variety of materials of your choice selectively into either cytoplasms or nuclei of adherent cell s . Inject into hundreds of cells per hour in a cell-context preserving , non-destructive and measurable manner. From nanoparticles to proteins, CRISPR RNA and plasmids, you can inject virtually any kind of liquid-based solutions containing particles below 500 nm in diameter. Success of microinjection using the FluidFM BOT has already been demonstrated in various kinds of cell types such as human cervical cancer cells (HeLa), human embryonic kidney cells (HEK) or human induced pluripotent stem cells (hiPS). Th e FluidFM BOT is a revolutionary tool for gene engineering, epigenetics, pharmaceutical drug development, toxicology, stem and cancer cell research, cell line development and many other cell and bio science research areas. As a stand-alone system and with a high level of automation and intuitive instrument operation, the FluidFM BOT is a remarkably powerful tool for s ing le cell biology. PRECISE CYTOPLASM OR NUCLEUS FAST 100+ CELLS / HOUR GENTLE 95%+ CELL VIABILITY The FluidFM μ3Dprinter offers truly new capabilities in manufacturing complex microscopic and submicroscopic metal structures. Micrometer- accurate 3D printing revolutionizes micro-manufacturing by combining additive manufacturing with traditional microfabrication methods. The FluidFM μ3Dprinter is a stand-alone system for printing metal objects at micrometer scale. The printed structure size is 1 μm 3 to 1’000’000 μm 3 , a range virtually inaccessible by other techniques. Practically any design can be printed by the system, including overhanging structures with 90 degree angles, without the need for support structures or post-processing steps. The unique technology of the FluidFM μ3Dprinter is capable of being industrially scalable. Additionally, the FluidFM μ3Dprinter also allows the dispensing of various liquids and nanoparticles. These capabilities lead to new research opportunities in fields such as microelectronics, semiconductors, surface modification, material science and many more. The FluidFM μ3Dprinter is the ideal system for metal micro-object manufacturing and for dispensing liquids as well as nanoparticles. BOT μ 3D printer MEASURABLE FEMTOLITER INJECTED VOLUMES PRECISE MICROMETER RESOLUTION REAL - T IME PROCESS C ONTROL VERSATILE VARIOUS MATERIALS FAST up to 100 μm/s ADD-ON 2 μm FluidFM ADD-ON. Empower your AFM. SPOTTING. One of many applications made possible by the FluidFM ADD-ON. FluidFM BOT. Stand-alone system for microinjection (incubator not shown). GENTLE MICROINJECTION. Repeatedly inject into the same living cell. FluidFM μ3Dprinter. Stand-alone system for printing metal objects at micrometer scale. PRINTED OBJECT. Printed in one single step and without support structures. 12+ APPLICATIONS 100% AFM CAPABILITIES MAINTAINED TAILORED AFM SOLUTION CUSTOMIZED SOLUTIONS POSSIBLE

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