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1. Subject to change without notice. Revision: 2.0 ID: CYTO-14-53 Copyright © 2016 Cytosurge AG, Switzerland FROM SINGLE CELL INJECTION TO MATERIAL SCIENCES. SELECTED F luid FM ® PUBLICATIONS MAIN PUBLICATION – A. Meister, M. Gabi, P. Behr, P. Studer, J. Vörös, P. Niedermann, J. Bitterli, J. Polesel - Maris, M. Liley, H. Heinzelmann & T. Zambelli. FluidFM: Combining atomic force microscopy and nanofluidics in a universal liquid delivery system for single cell applications and beyond. (2009) Nano Letters, 9 (6), 2501 – 2507. doi:10.1021/nl901384x 2016 – N. Helfricht, E. Doblhofer, J.F.L. Duval, T. Scheibel & G. Papastavrou. Colloidal Properties of Recombinant Spider Silk Protein Particles. The Journal of Physical Chemistry C, acs.jpcc.6b03957. doi:10.1021/acs.jpcc.6b03957 – Y. Lee, Y. Kim, D. Lee, D. Roy & J.W. Park. Quantification of Fewer Than Ten Copies of a DNA Biomarker without Amplification or Labeling. Journal of the American Chemical Society, jacs.6b02791. doi:10.1021/jacs.6b02791 – L. Jaatinen, E. Young, J. Hyttinen, J. Vörös, T. Zambelli & L. Demkó. Quantifying the effect of electric current on cell adhesion studied by single-cell force spectroscopy. Biointerphases, 11(1), 011004. doi:10.1116/1.4940214 – L. Hirt, S. Ihle, Z. Pan, L. Dorwling-Carter, A. Reiser, J.M. Wheeler, R. Spolenak, J. Vörös & T. Zambelli. Template-Free 3D Microprinting of Metals Using a Force-Controlled Nanopipette for Layer-by-Layer Electrodeposition. Advanced materials. doi:10.1002/adma.201504967 – J.S. McGrath, J. Quist, J.R.T. Seddon, S.C.S. Lai, S.G. Lemay & H.L. Bridle. Deformability Assessment of Waterborne Protozoa Using a Microfluidic-Enabled Force Microscopy Probe. PloS one, 11(3), e0150438. doi:10.1371/journal.pone.0150438 2015 – P. Roder & C. Hille. A Multifunctional Frontloading Approach for Repeated Recycling of a Pressure-Controlled AFM Micropipette. PLOS ONE, 10(12), e0144157. doi:10.1371/journal.pone.0144157 – D. Ossola, L. Dorwling-Carter, H. Dermutz, P. Behr, J. Vörös & T. Zambelli. Simultaneous Scanning Ion Conductance Microscopy and Atomic Force Microscopy with Microchanneled Cantilevers. Physical Review Letters, 115(23), 238103. doi:10.1103/PhysRevLett.115.238103 – L. Hirt, R.R. Grüter, T. Berthelot, R. Cornut, J. Vörös & T. Zambelli. Local surface modification via confined electrochemical deposition with FluidFM. RSC Adv., 5(103), 84517–84522. doi:10.1039/C5RA07239E – J. Geerlings, E. Sarajlic, E.J.W. Berenschot, R.G.P. Sanders, M.H. Siekman, L. Abelmann & N.R. Tas. Electric field controlled nanoscale contactless deposition using a nanofluidic scanning probe. Applied Physics Letters, 107(12), 123109. doi:10.1063/1.4931354 – D. Ossola, M. - Y. Amarouch, P. Behr, J. Vörös, H. Abriel & T. Zambelli. Force-controlled patch clamp of beating cardiac cells. (2015) Nano letters, 15 (3), 1743 – 50. doi:10.1021/nl504438z – E. Potthoff, D. Ossola, T. Zambelli & J. A. Vorholt. Bacterial adhesion force quantification by fluidic force microscopy. (2015) Nanoscale, 7 (9), 4070 – 4079. doi:10.1039/c4nr06495j – B. R. Simona, L. Hirt, L. Demkó, T. Zambelli, J. Vörös, M. Ehrbar & V. Milleret. Density gradients at hydrogel interfaces for enhanced cell penetration. (2015) Biomater. Sci. doi:10.1039/C4BM00416G – R. R. Grüter, B. Dielacher, L. Hirt, J. Vörös & T. Zambelli. Patterning gold nanoparticles in liquid environment with high ionic strength for local fabrication of up to 100 μm long metallic interconnections. (2015) Nanotechnology, 26 (17), 175301. doi:10.1088/0957-4484/26/17/175301 19 + TOPICS 6+ FIELDS 7+ Ø IMPACT FACTOR 11+ APPLICATIONS

2. CYTOSURGE AG, SÄGEREISTRASSE 25, 8152 GLATTBRUGG, SWITZERLAND PHONE +41 44 533 14 50, FAX +41 44 533 14 59, WWW.CYTOSURGE.COM CONTACT US. We offer complete support for our customers and distributors. Please visit the Cytosurge Help Center in order to access the FluidFM ® user community. www.fluidfm.com Subject to change without notice. Revision: 2.0 ID: CYTO-14-53 Copyright © 2016 Cytosurge AG, Switzerland 2014 – O. Guillaume - Gentil, E. Potthoff, D. Ossola, C. M. Franz, T. Zambelli & J. A. Vorholt. Force-controlled manipulation of single cells: From AFM to FluidFM. (2014) Trends in Biotechnology. Elsevier Ltd. doi:10.1016/j.tibtech.2014.04.008 – H. Dermutz, R. R. Grüter, A.M. Truong, L. Demkó, J. Vörös & T. Zambelli. Local polymer replacement for neuron patterning and in situ neurite guidance. (2014) Langmuir : the ACS journal of surfaces and colloids, 30 (23), 7037 – 46. doi:10.1021/la5012692 – E. Potthoff, D. Franco, V. D’Alessandro, C. Starck, V. Falk, T. Zambelli, J. A. Vorholt, D. Poulikakos & A. Ferrari. Toward a rational design of surface textures promoting endothelialization. (2014) Nano Letters, 14 (2), 1069 – 1079. doi:10.1021/nl4047398 – O. Guillaume - Gentil, T. Zambelli & J. A. Vorholt. Isolation of single mammalian cells from adherent cultures by fluidic force microscopy. (2014) Lab on a chip, 14 (2), 402 – 14. doi:10.1039/c3lc51174j – J. Geerlings, E. Sarajlic, J. W. Berenschot, R. G. P. Sanders, L. Abelmann & N. R. Tas. Electrospray deposition from AFM probes with nanoscale apertures. (2014) In MEMS 2014 (pp. 100 – 103). San Francisco: IEEE. Retrieved from http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=6765583 2013 – O. Guillaume - Gentil, E. Potthoff, D. Ossola, P. Dörig, T. Zambelli & J. A. Vorholt. Force-controlled fluidic injection into single cell nuclei. (2013) Small, 9 (11), 1904 – 1907. doi:10.1002/smll.201202276 – P. Schön, J. Geerlings, N. Tas & E. Sarajlic. AFM Cantilever with in Situ Renewable Mercury Microelectrode. (2013) Analytical chemistry, 85 (19), 8937 – 42. doi:10.1021/ac400521p – P. Stiefel, T. Zambelli & J. A. Vorholt. Isolation of optically targeted single bacteria by application of fluidic force microscopy to aerobic anoxygenic phototrophs from the phyllosphere. (2013) Applied and Environmental Microbiology, 79 (16), 4895 – 4905. doi:10.1128/AEM.01087-13 – P. Dörig, D. Ossola, A. M. Truong, M. Graf, F. Stauffer, J. Vörös & T. Zambelli. Exchangeable colloidal AFM probes for the quantification of irreversible and long-term interactions. (2013) Biophysical Journal, 105 (2), 463 – 472. doi:10.1016/j.bpj.2013.06.002 – R. R. Grüter, J. Vörös & T. Zambelli. FluidFM as a lithography tool in liquid: spatially controlled deposition of fluorescent nanoparticles. (2013) Nanoscale, 5 (3), 1097 – 104. doi:10.1039/c2nr33214k 2012 – E. Potthoff, O. Guillaume - Gentil, D. Ossola, J. Polesel - Maris, S. LeibundGut - Landmann, T. Zambelli & J. A. Vorholt. Rapid and Serial Quantification of Adhesion Forces of Yeast and Mammalian Cells. (2012) PLoS ONE, 7 (12), e52712. doi:10.1371/journal.pone.0052712 – P. Stiefel, F. I. Schmidt, P. Dörig, P. Behr, T. Zambelli, J. A. Vorholt & J. Mercer. Cooperative vaccinia infection demonstrated at the single-cell level using FluidFM. (2012) Nano Letters, 12 (8), 4219 – 4227. doi:10.1021/nl3018109 2010 – P. Dörig, P. Stiefel, P. Behr, E. Sarajlic, D. Bijl, M. Gabi, J. Vörös, J. A. Vorholt & T. Zambelli. Force-controlled spatial manipulation of viable mammalian cells and micro-organisms by means of FluidFM technology. (2010) Applied Physics Letters, 97 (2), 023701 1 – 3. doi:10.1063/1.3462979 2009 – A. Meister, J. Polesel - Maris, P. Niedermann, J. Przybylska, P. Studer, M. Gabi, P. Behr, T. Zambelli, M. Liley, J. Vörös & H. Heinzelmann. Nanoscale dispensing in liquid environment of streptavidin on a biotin-functionalized surface using hollow atomic force microscopy probes. (2009) Microelectronic Engineering, 86( 4-6), 1481 – 1484. doi:10.1016/j.mee.2008.10.025 – A. Meister, M. Gabi, P. Behr, P. Studer, J. Vörös, P. Niedermann, J. Bitterli, J. Polesel - Maris, M. Liley, H. Heinzelmann & T. Zambelli. FluidFM: Combining atomic force microscopy and nanofluidics in an universal liquid delivery system for single cell applications and beyond. (2009) Nano Letters, 9 (6), 2501 – 2507. doi:10.1021/nl901384x SELECTED F luid FM ® PUBLICATIONS

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