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1. CYTOSURGE AG, SÄGEREISTRASSE 25, 8152 GLATTBRUGG, SWITZERLAND PHONE +41 44 533 14 50, FAX +41 44 533 14 59, WWW.CYTOSURGE.COM SPOTTING FluidFM spotting enables you to reproducibly create high density arrays with femtoliter spot sizes, both in gaseous and liquid environ - ments. Print oligonucleotides, proteins, DNA, bacterial clones, oil and other materials with unpre- cedented precision and within their native environment. Applications of FluidFM spotting are found in life sciences, sensor technology, mini - mal lubrication and beyond. F luid FM ® GIVES YOU THE EDGE. FluidFM based spotting redefines vo- lumetric control of material spot printing. With FluidFM spotting it is possible to reliably produce spot sizes with volumes as low as a few femto- liters on a large variety of target sur - faces. The entire procedure can thereby be carried out in gaseous or liquid envi - ronment, with the option of additional humidity control. Precise adjustment of the relevant process parameters enables the creation of various spot sizes as required by your application. Create microarrays of numerous bio - molecules directly within their native SPOTTING. 1 Illustration of FluidFM nanopipette spotting. 2 Fluorescent image of femtoliter spots printed with FluidFM. Courtesy of Behr P., ETH Zurich environment to avoid denaturation of your sensitive samples. Create func - tionalized surfaces with unparalleled spatial resolution for the creation of novel biosensing devices with smaller footprints and higher levels of perfor - mance. Provide minimally lubricated surface modifications for your most demanding micromechanical compo - nents. FluidFM based spotting gives you the edge for your small scale material dispensing applications. THE PROCEDURE IN BRIEF. Spots are created while the FluidFM nanopipette is in contact with the tar- get surface via application of a short 1 2 FAST EXCHANGE OF INKS VERSATILE LIQUID & GAS ENVIRONMENT VAST CHOICE OF INKS PRECISE f L SPOT DISPENSING F luid FM ® – A WORLD OF APPLICATIONS. Subject to change without notice. Revision: 2.1 ID: CYTO-14-56 Copyright © 2016 Cytosurge AG, Switzerland pressure pulse generated by the Fluid- FM microfluidics control system. Interaction forces with the surface are thereby monitored and adjusted in real-time during the entire procedure. Reproducible control of spot size and dispensing volumes can be comfort- ably achieved via modification of the contact time and applied pressure. CONTACT US. We offer complete support for our customers and distributors. Please visit the Cytosurge Help Center in order to access the FluidFM ® user community. www.fluidfm.com SELECTED PUBLICATIONS – 2014. H. Dermutz, R. R. Grüter, A.M. Truong, L. Demkó, J. Vörös & T. Zambelli. Local poly - mer replacement for neuron patterning and in situ neurite guidance. Langmuir : the ACS journal of surfaces and colloids, 30 (23), 7037 – 46. doi:10.1021/la5012692 – 2009. A. Meister, J. Polesel - Maris, P. Nieder - mann, J. Przybylska, P. Studer, M. Gabi, P. Behr, T. Zambelli, M. Liley, J. Vörös & H. Heinzelmann. Nanoscale dispensing in liquid environment of streptavidin on a biotin-functionalized surface using hollow atomic force microscopy probes. Microelectronic Engineering, 86 ( 4-6), 1481 – 1484. doi:10.1016/j.mee.2008.10.025 20 μm

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