FluidFM® ADD‑ON for Nanosurf AFMs
To extend the capability of your Nanosurf AFM with FluidFM ADD-ON, contact our partner:
Extend the application scope of your Nanosurf atomic force microscope with our FluidFM ADD-ON. The FluidFM technology brings microfluidics to the tip of an AFM cantilever. The FluidFM ADD-ON is available for the Nanosurf DriveAFM, FlexAFM and CoreAFM platforms
Safe & reliable
The custom FluidFM probe holder for your AFM assures safe, tight and reliable mounting of FluidFM probes without the need of any tweezers.
Easy & fast probe mounting
The mounting of a FluidFM probe onto your AFM is fast and simple and does not require any tooling. The video (courtesy of Nanosurf) shows how a FluidFM probe is filled and mounted onto a Nanosurf FlexAFM.
Tailored to each system
Our FluidFM probe adapters are tailored to the geometrical and physical requirements of your Nanosurf AFM.
Use Cases with a FluidFM ADD-ON & Nanosurf AFMs
Mechanobiology
Colloidal spectroscopy of HeLa cells
A colloid is picked up by FluidFM and used to probe a living cell and create a force map. The FluidFM microfluidic control is fully integrated into the AFM software.
Left image: Map of Young's modulus (blue area is on glass).
Right image: Approach curves (force in nN versus tip-sample distance in µm) for the locations indicted with a star on the left: on glass (blue), harder cell areas (orange) and softer cell areas (black).
Image courtesy of Nanosurf AG.
Video courtesy of Nanosurf AG.
Cell adhesion measurements
Cell adhesion measurement at Harvard Center for Nanoscale Systems with a Nanosurf AFM.
Study of tumor progression and metastasis with cell-cell adhesion forces
Data/image courtesy of Tanya Konry group, Northeastern University, Boston, USA.
Study with a Nanosurf Flex-FPM by Dr. Noa Cohen, group of Prof. Tanya Konry, Northeastern University in Boston, on cell-cell adhesion forces to gain more insights into tumor progression and metastasis (Cohen et al., 2017):
Optical images showing:
A) a single cell to be picked up by a FluidFM probe
B) the cell aspired to the cantilever and
C) the FluidFM probe with aspired cell during a cell-cell adhesion measurement.
Data/image courtesy of Tanya Konry group, Northeastern University, Boston, USA.
A) Typical force curves between a MCF7 cell aspired to the cantilever and non-cancerous, fibroblast (HS5) on the substrate at different contact times.
B) Development of the force with contact time between the cells.
Nanoprinting
Create grids & patterns
Image courtesy of Nanosurf AG.
Deposit (bio)molecules and particles at defined locations with micrometer accuracy and with femtoliter volumes. A wide range of inks can be deposited in both air and liquid environment - from water to honey, solvents, acids, bases and more. For nanoscale and biomedicine research, biosensors and biomaterials. Automatically varying back pressure and contact time in a grid-like pattern (3 dots per condition), spot sizes can be quickly optimized.