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CONTINUOUS EXPERIMENTATION ON THE SAME LIVING CELL

FluidFM® OMNIUM

Powered by FluidFM technology, the OMNIUM platform brings nano-injection, Live-seq sampling, pick-and-place, adhesion measurement, bioprinting, isolation, force spectroscopy, and analysis into one continuous workflow on the same living cell.

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ModesWorkflowProbesApplicationsPerformanceOMNIUM SystemTechnical Specifications
Modes

Five modes. Same instrument. Same experiment.

Every mode operates on the same force-controlled probe, on the same identified cell.

INTRACELLULAR ACCESS

Nano-Injection

Deliver cargo directly into the cytoplasm of a single identified cell. Vector-free and force-controlled, nano-injection reaches unmatched precision without compromising viability, compatible with mRNA, plasmids, proteins, nanoparticles, and small molecules.

Up to 300 cells/hour · Vector-free delivery

Applications: Bioprinting · Growth factor patterning · ECM protein deposition · Spatial patterning · mRNA · Plasmids · Proteins · Nanoparticles · Small molecules

Sampling

Aspirate as little as 1 picoliter of cytoplasm from a single cell, then return the cell to culture. The cell survives and can be sampled again, enabling true longitudinal single-cell profiling without sacrificing the cell. Collects subcellular biopsies for Live-seq transcriptomics, metabolomics, and proteomics.

This is Live-seq, the underlying mechanism behind Causal Insights. See the science →

Applications: Live-seq · Metabolomics · Proteomics

CELLULAR ACCESS

Pick & Place

Select, isolate, and relocate individual cells to defined positions with image-verified precision. Ideal for isolating rare cells, constructing defined co-cultures, sorting organoids, or creating monoclonal cell lines, all with full cell viability.

Applications: Neuronal networks · monoclonal cell line creation · cell isolation · organoid sorting

Single-Cell Adhesion

Measure adhesion forces between individual cells and substrates with nanonewton precision. Quantify how cells interact with surfaces, ECM proteins, or other cells in real time on the same instrument, without changing your setup.

Up to 200 cells/day · Nanonewton precision

Applications: Mechanobiology · Metastatic potential · Drug screening · ECM characterisation

Dispensing & Nanoprinting

Deposit fL–nL volumes in defined patterns. From 300 nm features to cm-scale areas, OMNIUM's dispensing mode enables submicron-resolution bioprinting and spatial patterning of biomolecules, including growth factors, ECM proteins, and living cells.

Sub-µm resolution · 2.5D capable

Applications: Bioprinting · growth factor patterning · ECM protein deposition · spatial patterning
Workflow

WORKFLOW

From sample to result in a single sessions

FluidFM OMNIUM ships as one integrated experimental platform. Imaging, environmental control, FluidFM instrumentation, operator software, and data management work together for longitudinal live cell workflows.


Learn more about FluidFM Technology

01

Prepare

Load your plate and select probes and targets in ARYA's plate editor, no manual calibration between runs.

02

Point & click

Select the exact cell you want under the microscope. Force feedback finds the membrane and controls penetration automatically.

03

Analyze & export

Review viability and yield in real time, then export structured data straight into your existing pipeline.

Probes

Three probes, one platform

Swap probes without recalibrating the system. Each ships with a QR code linking to its spec sheet and a unique SEM image. A prototyping probe with a closed or custom aperture available on request.

Micropipette

2, 4, or 8 µm flat circular aperture.

  • Cell Isolation
  • Pick-and-Place
  • Adhesion Measurement

Nanosyringe

600 nm pyramidal side aperture.

  • Live Cell Injection
  • Cytoplasmic Sampling

Nanopipette

S300 nm aperture.

  • Spotting
  • Nanoprinting
  • Sub-micron Particle Manipulation
Applications

MULTIPLE SAME-CELL WORKFLOWS

One instrument for live-cell manipulation, measurement, and analysis

Use FluidFM OMNIUM across live-cell transcriptomics, vector-free delivery, cell isolation, force spectroscopy, and bioprinting workflows.

Live-seq

Repeated live-cell transcriptomics from the same living cell across time. Sample, perturb, and re-sample while preserving cell viability.

Learn more about Live-seq →

Vector-free cargo delivery

Deliver mRNA, proteins, CRISPR reagents, or nanomaterials directly into living cells without viral vectors or electroporation.

Learn more about CellEDIT →

Single-cell pick & place

Select, relocate, and organize individual living cells with image-verified precision for array generation, co-culture design, and population sorting.

Single-cell isolation

Isolate individual living cells for downstream analysis, monoclonal expansion, and image-verified single-cell workflows.

Automated force spectroscopy

Quantify cell adhesion, stiffness, and mechanical response at single-cell resolution with automated force measurements across large experimental datasets.

Bioprinting

Pattern biomolecules and living cells in defined spatial arrangements from sub-micron features to centimetre-scale constructs.

Performance

Precision engineered for continuous live-cell workflows

Nanometer-scale positioning, pressure control, environmental stability, and live-cell imaging in one integrated platform.

< 500 nm

XY repeatability

Highest positioning precision for cell and probe tracking.

< 5 nm

Z repeatability

Nanometer-precise vertical positioning for controlled intracellular access.

-800 to +1,000 mbar

Pressure range

Full range for injection, aspiration, and suction in a single system.

0.5 mbar

Pressure resolution

Fine-grained pressure control for repeatable injection volumes.

± 0.1°C

Temp​erature

Physiological temperature precision for cell viability throughout the experiment.

1–15%

CO₂ control

± 0.1% accuracy for stable incubation across extended live-cell sessions.

OMNIUM System

INTEGRATED SYSTEM

FluidFM OMNIUM integrates hardware, software, and workflows in one platform

FluidFM OMNIUM integrates live-cell imaging, FluidFM instrumentation, environmental control, software, and data management in one system for longitudinal single-cell experiments.

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Included with every OMNIUM system:

  • Installation and site qualification
  • Integrated microscope, FluidFM head, incubation system, and anti-vibration table
  • Brightfield and epi-fluorescence imaging with automated focus correction
  • ARYA operator software and EDNA data management platform
  • FluidFM starter probe kit (Micropipette, Nanosyringe, Nanopipette)
  • Application training and workflow onboarding
  • Preventive service coverage

ARYA software and workflow automation:

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Streamlined workflows

Plate editor, probe library, and run queue in one interface.

Cell tracking & navigator

Track and revisit the same identified cell across repeated sessions.

Consumable management & automated washing

Track probe stock and usage automatically per run.

Quarterly software updates

New protocols and probe profiles released every quarter.

Technical Specifications

SYSTEM DETAILS

Specifications at a glance

Compare key OMNIUM performance metrics or explore the complete system specifications, including microscopy, fluorescence, incubation, FluidFM control, and sample handling.


 Key performance metrics.

Metric Value Why it matters
XY repeatability < 500nm Highest positioning precision for cell and probe tracking.
Z repeatability < 5 nm Nanometer-precise vertical positioning for controlled intracellular access.

Pressure range

-800 to +1,000 mbar

Full range for injection, aspiration, and suction in a single system.

Pressure resolution

0.5 mbar

Fine-grained pressure control for repeatable injection volumes.

Temperature

± 0.1°C

Physiological temperature precision for cell viability throughout the experiment.

CO₂ control

1–15%, ± 0.1%

Physiological CO₂ maintained from setup through final data export


 Complete OMNIUM full system specifications.

Metric Spec Why it matters

Microscopy

Objectives

10x, 20x, 40x long-distance


Imaging

Brightfield


Configuration

Customizable


Focus stroke / increment

10.5 mm stroke, 0.01 µm increment

Fluorescence

Light source

White LED, 360–770 nm, 25,000 h lifetime


Filter sets

3 standard, Red, Green, Blue, expandable to 8


Filter switch

300 ms, motorized turret

Camera

Sensor

CMOS Color, 2.35 MP, 12-bit, 2nd camera option available

Incubation

Temperature

37°C ± 0.1°C


CO₂

1–15%, ± 0.1% accuracy


Sterility

HEPA filter + UV sterilization


Casing

Polycarbonate, optimized for fluorescence imaging

FluidFM

Pressure

−800 to +1,000 mbar, 0.5 mbar resolution


Stage

Nanometer-precise XY + Z, < 500 nm / < 5 nm repeatability


Probe exchange

Motorized, automated

Sample handling

Containers

Multi-well plates, up to 2x standard, dishes, glass-bottom dishes

Sample Compatibility: Your standard cell culture ware fits the system. No special consumables needed.

Download Full Specifications PDF


Expand Your System: Modular expansion with application-specific probes, consumables, and software.

Explore the Probe Portfolio       

A proven foundation for Same-Cell biology

Built for leading academic, biotech, and pharma teams.

140+

FluidFM installations worldwide

160+

Peer-reviewed publications

7

Patent families, key patents to 2037

SAME-CELL MOLECULAR PROFILING

Causal Insights

Causal Insights

Access longitudinal same-cell sequencing without running the workflow in-house. Cytosurge performs the experiment and delivers the resulting data.

Explore Causal Insights →

IMPOSSIBLE GENETIC EDITS

CellEDIT

CellEDIT

Access FluidFM-enabled genome engineering for complex edits and hard-to-transfect cell lines without building the workflow internally.

Explore CellEDIT →


Your OMNIUM questions, answered

Find answers to common questions about the FluidFM OMNIUM platform, from lab requirements to application-specific details.

The FluidFM OMNIUM is a standalone, automated single-cell manipulation system combining atomic force microscopy with microfluidics. It enables gentle extraction and injection at the single-cell level using force-controlled FluidFM probes with apertures as small as 600nm, maintaining 85-89% cell viability.

The FluidFM OMNIUM supports five core application modes. Nano-injection delivers any cargo — plasmids, CRISPR reagents, nanoparticles, viruses — into individual cells with cytoplasm or nucleus selectivity. Sampling collects subcellular biopsies for Live-seq, metabolomics, and proteomics.


Pick and Place selects and isolates living cells for monoclonal lines, neuron networks, and organoid sorting. Adhesion quantifies cell-substrate and cell-cell adhesion forces for mechanobiology. Bioprinting enables bio-patterning and axon guidance from image or file.

Three standard probes are available. The FluidFM Nanosyringe (600 nm pyramidal side aperture) handles nano-injection and cytoplasmic or nuclear extraction. The FluidFM Micropipette (2, 4, or 8 μm flat circular aperture) is designed for cell isolation, pick-and-place, and adhesion measurements.


The FluidFM Nanopipette (300 nm aperture) is used for spotting, nanoprinting, and sub-micron particle manipulation. A Prototyping Probe with a closed or custom aperture is also available. All probes ship with a QR code linking to specifications and a unique SEM image.

The system requires a dedicated footprint of 1.5 m × 1.5 m, with a total recommended lab space of 4 m² (43 sq ft) to allow cleaning and maintenance access. Total system weight is up to 500 kg.


Power requirements are 13 plugs on the same ground (600 W average, 2500 W peak). A fast, stable Ethernet connection is required for setup and remote support. The lab must maintain 18–37 °C, 20–65% relative humidity, and 950–1100 hPa atmospheric pressure. Installation will not proceed if requirements are not met in advance.

The platform is validated for 2D cultures of adherent mammalian cells for biopsy applications. For cell isolation, both adherent cells (via local trypsinization) and suspension cells (via suction at −100 mbar) are supported.


Compatible formats include 6, 12, and 24 well plates; 50 mm WillCo and Mattek dishes; and microscopy slides 0.5–1.5 mm thick. Custom formats can be configured using the ARYA Plate Editor.

Standard purchase includes free software updates for 12 months and complimentary EDNA data management software. Cytosurge also offers the 360 Support Package, which adds care-free service agreements, multi-year warranty extension, trimestral ARYA software releases, and premium support ranging from training to experiment design and result evaluation. Both on-site and remote experimental support are available post-installation

OMNIUM is semi-automated: you select the target cell under the microscope, and the system's force feedback handles the approach, penetration, and retraction automatically. Once a plate is queued in ARYA, runs proceed across many cells without re-calibration between them.

Download the full specifications above, or book a demo and our applications team will walk through OMNIUM on your own workflow.

Not every workflow requires bringing OMNIUM into your lab. Cytosurge also​ provides specialized services built on FluidFM technology. 

See CellEDIT or Causal Insights for more information.


Ready to bring FluidFM OMNIUM into your lab?

The FluidFM OMNIUM platform is ready for your lab. Design the experiment your biology demands, not the one your instruments constrain.

Talk to a Expert