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precisION CRISPR CELL LINE ENGINEERING

CellEDIT

Impossible Genetic Edits.

Made Possible.


Engineer what cells become with CellEDIT, combining FluidFM® precision, direct intranuclear CRISPR delivery, and expert genome engineering to create validated monoclonal cell lines beyond the limits of conventional transfection.

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CellEDIT is built for difficult genome engineering projects where conventional delivery methods fall short. It combines precise intranuclear CRISPR delivery with expert design, screening, and validation to improve access to hard-to-transfect cell lines and complex edits. The result is a more controlled path from edit design to validated monoclonal clones.

Precision delivery

Deliver CRISPR cargo directly into individual cells with controlled dosage and minimal disruption.

Higher first-pass success

Reduce optimization cycles with a workflow designed for difficult-to-transfect cell lines.

Validated clonal results

Receive engineered monoclonal cell lines confirmed through sequencing and project-specific quality controls.

FluidFM® Technology

FLUIDFM NANOSCALE PRECISION

Precision delivery. No compromises.

FluidFM is Cytosurge's proprietary intracellular delivery technology. A hollow cantilever thinner than a cell delivers CRISPR editing machinery directly into the nucleus with precise dosage control, eliminating the variability associated with bulk delivery methods.


Learn more about FluidFM technology

Stoichiometric delivery

Exact, controlled injection of RNPs and donor templates into every single cell.

Maximized editing specificity

Precisely controlled reagent concentrations prevent the over-dosing seen in bulk delivery, drastically reducing off-target effects.

Uncompromised cell integrity

Unlike harsh electroporation, only FluidFM preserves the native physiological and morphological state of your cells.

Physiological relevance

You are engineering a model. Toxic delivery methods create survivor cells that no longer faithfully represent the biology you are studying.

Downstream data inte​grity

Preserves biologically relevant cell models for more reliable downstream assays.

Guaranteed monoclonality

Every clone is derived from a single, visually verified cell. Our dual image based monoclonality proof satisfies regulatory requirements.

CellEDIT vs. Traditional Methods

WHY CELLEDIT

CellEDIT vs. traditional gene editing approaches

Capability CellEDIT (FluidFM) Electroporation Lipofection Viral
Dose Control Precise (pg level) None None
Hard-to-transfect Cells Success High Low None Moderate
Multiplex Reliability High Low Low Moderate
Vector-Free Option Yes Yes Yes No
Nuclear Targeting Yes No No Yes
CellEDIT Workflow

CELLEDIT WORKFLOW

How CellEDIT projects move from design to validated clones


Our gene editing service handles the complexity of sensitive cells so you can focus on downstream research. This standardized, reproducible workflow bypasses biological barriers.

Consultation

Project feasibility and CRISPR design strategy

Cell Pickup

Cell Packaging & Collection

CRISPR Design

Development of an editing strategy

CRISPR Injection

FluidFM delivery of CRIPR RNPs into single cells

Clonal Outgrowth

Single cell seeding and monoclonal outgrowth

Screening

Genotypic screening of targeted edits

Quality Control

Monoclonality, viability, sterility, and mycoplasma testing

Shipment

Cryopreserved clones and project report delivery

Deliverables
CellEDIT team gene engineering

PROJECT DELIVERABLES

What's included in a CellEDIT project?

Every CellEDIT project combines expert genome engineering support with validated clones and the documentation needed to move confidently into downstream research

Versatile Image
Edited monoclonal clones

Validated clones carrying the intended genetic modification.

Versatile Image
Wild-type control clones

Matched controls for downstream experimental comparison.

Versatile Image
gRNA design support

Guide RNA design and selection tailored to the intended edit.

Versatile Image
Validation report

Comprehensive documentation including sequencing results, monoclonality evidence, and project-specific quality-control data.

CellEDIT Solutions

GENE EDITING TYPES

Explore CellEDIT solutions


From precise gene knockouts to complex multiplex edits, CellEDIT provides validated genome-engineering solutions for difficult-to-edit cell lines.

Talk to a CellEDIT expert

Knockout

Knockout

Loss of function edits generated through intra-nuclear CRISPR RNP delivery and confirmed by clonal sequencing of both alleles. 

Explore Knockouts →


Multiplex gene editing

Multiplex gene editing

Simultaneous editing of three or more genes within a single Lacun-3 clone. Each target site is independently verified through clonal sequencing prior to release. 

Explore Multiplex gene editing →




Knock-in

Knock-in

Defined insertions up to approximately 120 bp without plasmid or viral integration. Edits are validated by sequencing.

Explore Knock-ins →

Large fragment excision

Large fragment excision

Domain or multi-exon deletions up to 4 kilobases, validated by junction PCR to confirm intended deletions without unintended rearrangements. 

Talk to a CellEDIT expert →

CellEDIT Solutions

EDIT TYPES

Explore CellEDIT solutions

From precise gene knockouts to complex multiplex edits, CellEDIT provides validated genome-engineering solutions for difficult-to-edit cell lines.

Knockout

Loss of function edits generated through intra-nuclear CRISPR RNP delivery and confirmed by clonal sequencing of both alleles.

Explore Knockouts →

Knock-in

Defined insertions up to approximately 120bp without plasmid or viral integration. Edits are validated by sequencing. 

Explore Knock-ins →

Multiplex gene editing

Simultaneous editing of three or more genes with a single Lacun-3 clone. Each target site is independently verified through clonal sequencing prior to release. 

Explore Multiplex gene editing →

Large fragment excision

Domain or multi-exon deletions up to 4 kilobases, validated by junction PCR to confirm intended deletions without unintended rearrangements.

Talk to a CellEDIT expert →

FAQs

CellEDIT FAQs

Find answers project planning, validation, timelines, supported cell types, pricing, and the overall workflow.

We’ve successfully edited diverse cell types including many considered “difficult”. Rather than make assumptions, lets discuss your specific application. We can often provide feasibility data form similar projects or propose a small pilot to demonstrate success before committing to a full project.

Cells can be provided by the customer and sent directly to us. Cells received from customers must pass internal validation to ensure they are suitable for our process. Alternatively, cells can be ordered from official cell providers such as ATCC and shipped directly to us.

Vector-free delivery minimizes random genomic integration and supports precise, controlled editing, which is beneficial for sensitive cell types and complex engineering tasks.

FluidFM technology has been developed over 15+ years with extensive peer-reviewed publications. Cytosurge has successfully completed numerous customer projects across pharma and biotech. We’re happy to provide references from existing customers and share detailed technical data demonstrating performance in specific cell types relevant to your application.

CellEDIT projects are tailored to your research objectives, so timelines vary depending on the edit design, cell type, and validation requirements. Speak with a CellEDIT expert to discuss your project and receive a project-specific timeline.

Project pricing depends on factors such as the selected cell line, edit type, number of targets, and validation requirements. Final pricing is confirmed during a consultation following feasibility review.


START YOUR PROJECT

Your research goals. 

Our cell line engineering expertise.

Discuss your target cell line, edit strategy, and validation requirements with our CellEDIT team.