Overview
Live-seq pairs a non-destructive cytoplasmic biopsy with downstream sequencing, so you can read a cell's transcriptome and keep tracking it afterward. Start here for the big picture before diving into a specific step.
Cell line onboarding
Before your first Live-seq run, characterize your cell line's baseline properties: RNA content, viability, seeding density, and imaging settings, so your protocol is calibrated to your model system.
Feasibility biopsy
Set up your workflow and confirm biopsies are feasible on your cells: preparation, the step-by-step procedure, and how to assess viability afterward.
Clean biopsy
Avoid cross-contamination between samples with a validated clean-biopsy collection technique.
Viability & Sequential biopsy
Confirm your cells survive the first extraction, then re-biopsy the same living cell over time to build a real trajectory, not a population average.
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