Discover CRISPR Gene Editing by CellEDIT
Our workflow delivers CRISPR complexes directly into the nucleus, providing an effective CRISPR Gene Editing.
CRISPR gene editing precisely cuts DNA, leveraging natural repair mechanisms to modify specific genome locations. [1,2] This adaptability of CRISPR-Cas9 has ignited rapid advancements in diverse fields, from synthetic biology and human gene therapy to neuroscience and agriculture.
While several gene editing techniques exist, each faces the challenge of efficiently accessing the nucleus to modify the genome without harming the cell. Though CRISPR excels in scenarios needing multiple edits within a single cell line or organism, it sometimes falls short due to low HDR efficiency and off-target effects.
Presently, the CellEDIT workflow with the FluidFM technology enhances CRISPR's efficiency and versatility in various cell types and is pivotal for cell line engineering and development.
Vector-free Gene Editing by direct intra-nuclear delivery.
Our workflow directly injects CRISPR reagents into the nucleus, removing the need for carriers or vectors and bypassing template size limitations.
Suited for Hard-to-Transfect Cells.
CellEDIT's gentle injection workflow uses FluidFM to deliver CRISPR complexes directly into the nucleus, suited for developing cell lines in hard-to-transfect cells.
Minimized Off-Targets
Direct intra-nuclear injection delivers all CRISPR components simultaneously and at the correct concentration, effectively minimizing off-target effects.
Efficient & Gentle Workflow
Save time and resources in the lab with a personalized gene editing service based on our efficient and gentle injection workflow.
CRISPR Gene Editing - Case Studies
Experience the efficiency of the CellEDIT workflow firsthand. Download our application notes and delve into compelling case studies that demonstrate its transformative impact on immortalized and cancer cell lines.
Cancer Cell Lines
Discover how the CellEDIT workflow achieved 3 monoclonal HPRT1 knockouts in the notoriously hard-to-transfect SK-MES-1 cell line.
Immortalized Cell Lines
Explore how the CellEDIT workflow generated 5 monoclonal Hprt knockouts in the C2C12 cell line by directly injecting only 51 cells.
References
[1] Jinek, Martin, et al. "A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity." science 337.6096 (2012): 816-821.
[2] Li, Hongyi, et al. "Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances and prospects." Signal transduction and targeted therapy 5.1 (2020): 1-23.