A Unique Mammalian Cell Transfection Protocol
Current Challenges in Mammalian Cell Transfection
The successful transfection of cells poses significant challenges, stemming from the intricate nature of the process and the multiple steps involved in introducing external nucleic acids, peptides, or proteins into eukaryotic cells. [1-3] Particularly when dealing with mammalian cell transfection methods, two crucial hurdles must be surmounted.
Amidst the array of existing cell transfection techniques, the single-cell transfection approach utilizing the FluidFM technology stands out for its exceptional merits. This method not only presents a comprehensive solution to circumvent substantial limitations but also offers a set of distinctive advantages for each step of the transfection process.
Step 1: Pass the Cell Membrane & Avoid Random Transport, Cytosolic Degradation
The revolutionary single-cell transfection method offered by the CellEDIT workflow introduces a groundbreaking capability to deliver precise and highly controlled quantities of CRISPR Cas9 Ribonucleoproteins directly into the nucleus. By confining the DNA cutting process exclusively within the nucleus, this technique effectively sidesteps the potential activation of immune responses and cytosolic degradation. As a result, the efficiency of this method surpasses that of conventional approaches, maximizing the desired outcomes with unparalleled precision.
Step 2: Pass the Nuclear Envelope & Deliver
Distinguished from conventional cell transfection methods, the CellEDIT workflow employs a remarkable transfection technique rooted in the innovative FluidFM technology. This cutting-edge approach accomplishes the delivery of Ribonucleoproteins (RNPs) directly into the nucleus through precise nano-injection, effectively eliminating an entire step from the experimental process. By adopting this bottom-up strategy, the number of procedural steps involved in cell transfection is significantly reduced. Furthermore, since the injection is conducted on individual isolated cells, the need for laborious cell sorting procedures is entirely eliminated. This streamlined methodology streamlines the workflow and enhances the overall efficiency of the transfection process.
Step 3: Perform Step 1 & 2 while keeping the cell alive
Unlock the potential of your research endeavors with the groundbreaking cell transfection approach offered by the CellEDIT Service. This innovative methodology prioritizes cell viability by employing a non-destructive and meticulously controlled technique to deliver CRISPR complexes directly into the nucleus. Whether your objective is gene knock-in, gene knock-out, or multiplexing, this cutting-edge workflow caters to a diverse array of applications. With CellEDIT Service, you can confidently embark on your scientific journey, knowing that you have access to a revolutionary tool that enhances precision and expands the possibilities of genetic manipulation.
 Chong, Zhi Xiong, Swee Keong Yeap, and Wan Yong Ho. "Transfection types, methods and strategies: A technical review." PeerJ 9 (2021): e11165.
 Kim & Eberwine (2010) Kim TK, Eberwine JH. Mammalian cell transfection: the present and the future. Analytical and Bioanalytical Chemistry. 2010;397(8):3173–3178. doi: 10.1007/s00216-010-3821-6.
 Fus-Kujawa, Agnieszka, et al. "An overview of methods and tools for transfection of eukaryotic cells in vitro." Frontiers in Bioengineering and Biotechnology (2021): 634.