Custom Cell Line Engineering - Use Cases
Let CellEDIT handle your custom cell line engineering, so you can focus on your research.
Custom Cell Line Engineering by CellEDIT
The CellEDIT service leverages the unique advantages of the FluidFM® technology to create new ways of engineering cells. We draw inspiration from what researchers need most:
- High-quality disease models they can trust for their research.
- A new CRISPR approach which surpasses current possibilities, enabling biomedical research to thrive.
The CellEDIT Service Workflow with a 10-week delivery time.
Because every researcher has specific needs, we developed a service that adapts and responds best to each demand, from editing complexity to cell line-specific genotyping solutions and quality controls.
Effective communication was crucial for our project's success and prompt execution. Cytosurge’s scientific expertise and quick responses saved us time and prevented errors. This level of service was unmatched by other companies, where we encountered delays and limited scientific knowledge.
Custom Engineering of Immortalized Cell Lines
Discover how the CellEDIT workflow was used to efficiently generate to knockout HPRT in C2C12 cells, a housekeeping gene responsible for the maintenance of cellular function, to obtain 5 edited monoclonal cell lines.
Key findings:
- The CellEDIT workflow was employed to successfully generate 5 monoclonal HPRT knockouts in C2C12 cell line through direct intranuclear injection of only 51 cells.
- Genotype analysis revealed that 88% (36) of the expanded 41 clones were edited at the target site, with 41.5% (17) of the clones displaying editing on all alleles.
Custom Editing of Hard-to-Transfect Cell Lines
Explore how the CellEDIT workflow facilitated the production of 3 monoclonal HPRT1 knockouts in SK-MES-1 cells. This cell line, originating from squamous cell carcinoma, is known to be notoriously hard-to-transfect due to its high resistance to the uptake of foreign DNA and the typically low efficiency of common transfection methods.
Key findings:
- Genotype analysis of 22 clones revealed that 55% (12) of these were edited at the target site and 23% (5) of all clones displaying editing on all four alleles.
- Gentle delivery of CRISPR-RNPs through intranuclear injection ensured a survival rate of 65% in SK-MES-1 cells, thereby showcasing the highly controllable and gentle nature of the CellEDIT workflow and its potential for editing notoriously hard-to-transfect cells.
Custom Multiplex Gene Editing
At CellEDIT, we edit for you, multiple genes in a single experiment, providing you with multiplexed cell lines in 10 weeks.
The ease and efficiency of CellEDIT's vector-free editing by intra-nuclear delivery, makes it an ideal system to knockout one, two, or three target genes in one go. Find how the CellEDIT workflow boosted gene editing efficiency while preserving cell characteristics and functionality in single and multiple knockouts CHO-K1 clones. The CHO cell line is derived from an ovarian biopsy of an adult female Chinese hamster in 1957, is widely used in pharmaceutical and therapeutic protein production.
The editing strategy was challenging due to overlapping regions and the need for a precise knockout to ensure the gene was completely shut down. Cytosurge achieved this with accuracy. The results met our expectations confirming our hypothesis and facilitated the successful revision of a paper.
Key findings:
- The control of the amount and stoichiometry of RNP complexes could boost efficiency, particularly when targeting multiple loci simultaneously.
- The preservation of general cell characteristics and functionality post-editing in both single and multiple KO clones in CHO-K1 cells was demonstrated.
Related Resources
Technical
Introduction to CRISPR Knockout Gene Editing with CellEDIT
An Overview of CellEDIT' CRISPR Cell Line Development Services
CellEDIT' CRISPR Cell Line Development Workflow
CellEDIT's Engineered Cell Lines
CRISPR-Engineered U2OS Cell Line
CRISPR-Engineered MDA MB 231 Cell Line
CRISPR-Engineered A549 Cell Line
CRISPR-Edited Hek293 Cell Line
CRISPR-Engineered C2C12 Cell Line
Media & Downloads
On-Demand CellEDIT's CRISPR Cell Line Engineering Webinar
CellEDIT's Application Note n*1 - Introducing the CellEDIT Workflow
CellEDIT's Application Note n*2 - Overcoming the hard-to-transfect cell line hurdle
Open Access Publication featuring CellEDIT: Antony, Justin S., Anabel Migenda Herranz, Tahereh Mohammadian Gol, Susanne Mailand, Paul Monnier, Jennifer Rottenberger, Alicia Roig‐Merino et al. " Accelerated generation of gene-engineered monoclonal CHO cell lines using FluidFM nanoinjection and CRISPR/Cas9" Biotechnology Journal 19, no. 4 (2024): 2300505.