The discovery of CRISPR-Cas9 for targeted gene engineering has revolutionized life sciences. Regardless of its broad introduction across many disciplines in biology, several challenges persist in editing cell lines, particularly for sensitive cells, or when multiple edits are required. FluidFM offers a unique in-vitro precision gene editing capability to improve the efficiency and applicability of CRISPR across a variety of cell types and for cell line development. In this application note, we provide an overview of the extensive functionalities FluidFM technology offers for CRISPR Cell Line Engineering.