CRISPR Knockout Cell Line Engineering - Application note
The CellEDIT Workflow Facilitates the Efficient Production of Knockout Clonal Cell Lines by Direct Intranuclear Injection
The CellEDIT workflow successfully generated 4 monoclonal hprt knockouts in C2C12 cell line by direct intranuclear injection of only 27 cells.
Genotype analysis revealed that 97% of the expanded clones presented gene editing, with 48% of the clones displaying editing on all alleles.
Direct intranuclear injection proofs to be a highly controllable and gentle alternative transfection method for CRISPR-mediated gene editing.