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CellEDIT CRISPR Cell Line Engineering Workflow - Application note

 

Key findings

  1.  The CellEDIT workflow is based on direct nuclear injection of RNP complexes into single cells using FluidFM™.
  2. The CellEDIT workflow was employed to successfully generate 5 monoclonal Hprt knockouts in C2C12 cell line through direct intranuclear injection of only 51 cells. 
  3. Genotype analysis revealed that 88% (36) of the expanded 41 clones were edited at the target site, with 41.5% (17) of the clones displaying editing on all alleles.


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