CRISPR Knockout Cell Line Engineering - Application note
The CellEDIT Workflow Facilitates the Efficient Production of Knockout Clonal Cell Lines by Direct Intranuclear Injection
Key findings
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The CellEDIT workflow successfully generated 4 monoclonal hprt knockouts in C2C12 cell line by direct intranuclear injection of only 27 cells.
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Genotype analysis revealed that 97% of the expanded clones presented gene editing, with 48% of the clones displaying editing on all alleles.
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Direct intranuclear injection proofs to be a highly controllable and gentle alternative transfection method for CRISPR-mediated gene editing.