Key findings

1.  The CellEDIT workflow is based on direct nuclear injection of RNP complexes into single cells using FluidFM™.
2. The CellEDIT workflow successfully generated 5 monoclonal Hprt knockouts in C2C12 cell line through direct intranuclear injection of only 51 cells. 
3. Genotype analysis revealed that 88% (36) of the expanded 41 clones were edited at the target site, with 41.5% (17) of the clones displaying editing on all alleles.