Key findings

1. The CellEDIT workflow was used to successfully generate 3 monoclonal HPRT1 knockouts in SK-MES-1 cell, a notoriously hard-to-transfect cell line.
2. Genotype analysis of 22 clones revealed that 55% (12) of these were edited at the target site and 23% (5) of all clones displaying editing on all four alleles.
3. Gentle delivery of CRISPR-RNPs through intranuclear injection ensured a survival rate of 65% in SK-MES-1 cells, thereby showcasing the highly controllable and gentle nature of the CellEDIT  workflow and its potential for editing notoriously hard-to-transfect cells.