CellEDIT | CRISPR Cell Line Development Workflow
Overview | CRISPR Cell Line Engineering Workflow
Amid advancements in technology and biopharmaceutical research, cell line development has gained prominence, particularly in drug discovery processes such as pathway analysis, target validation, and disease modeling. The surge in novel therapeutics has escalated the demand for superior cell line development. To address this, researchers have increasingly adopted CRISPR/Cas complexes alongside traditional transfection methods for cell line development. Traditional approaches rely on batch transfection methods like electroporation or lipofection. However, the CellEDIT service introduces a disruptive single-cell transfection workflow that delivers precise quantities of CRISPR Cas9 Ribonucleoproteins directly into the nucleus. This targeted approach minimizes the risk of immune response activation and cytosolic degradation, thus significantly enhancing efficiency and precision compared to conventional methods.
Cell Line Development Workflow by Direct Intranuclear Delivery.
For the successful commercialization of your product, an optimized, high-quality cell line development workflow is not just beneficial—it's essential. By outsourcing your cell line development to a specialized partner like Cytosurge, you leverage industry expertise, minimize risks, optimize results, and enhance operational flexibility. The CellEDIT Service offers a comprehensive, reliable workflow tailored for a variety of genome edits, including knockouts, knock-ins, and large insertions. Initial phases of our engagement involve rigorous onboarding of your cell line and injection optimization—critical preparatory steps before deploying the CellEDIT workflow. Once we've intimately understood the unique attributes of your cell line, the specialized CellEDIT process begins. Utilizing cutting-edge single-cell transfection technology, we inject CRISPR reagents directly into the nuclei of individual cells. The workflow concludes only upon successful clone editing, ensuring a highly customized outcome aligned with your specific requirements. Our technology significantly mitigates off-target effects by delivering precise volumes of RNP complexes directly into the nucleus, resulting in the reliable creation of high-quality engineered cell lines with unparalleled precision and control.
When it comes to the commercialization of your product, an effective, high-quality cell line development workflow is essential for your success. It requires a partner that provides you with experience, expertise, and a comprehensive portfolio of products and services — someone who can minimize risks, optimize outcomes and increase your flexibility.
The CellEDIT workflow begins with the injection of CRISPR reagents into a single cell. This meticulous procedure only concludes upon successful acquisition of edited clones, ensuring optimal results tailored to your specific needs.
When it comes to the commercialization of your product, an effective, high-quality cell line development workflow is essential for your success. It requires a partner that provides you with experience, expertise, and a comprehensive portfolio of products and services — someone who can minimize risks, optimize outcomes and increase your flexibility.
The CellEDIT workflow begins with the injection of CRISPR reagents into a single cell. This meticulous procedure only concludes upon successful acquisition of edited clones, ensuring optimal results tailored to your specific needs.
When it comes to the commercialization of your product, an effective, high-quality cell line development workflow is essential for your success. It requires a partner that provides you with experience, expertise, and a comprehensive portfolio of products and services — someone who can minimize risks, optimize outcomes and increase your flexibility.
The CellEDIT workflow begins with the injection of CRISPR reagents into a single cell. This meticulous procedure only concludes upon successful acquisition of edited clones, ensuring optimal results tailored to your specific needs.
To offer the most efficient and accurate editing service, preliminary steps involve the onboarding of your cell line and injection optimization; these are crucial prerequisites before initiating the CellEDIT workflow. Once we have familiarized ourselves with the unique characteristics of your cell line, we can then commence the CellEDIT process.
To offer the most efficient and accurate editing service, preliminary steps involve the onboarding of your cell line and injection optimization; these are crucial prerequisites before initiating the CellEDIT workflow. Once we have familiarized ourselves with the unique characteristics of your cell line, we can then commence the CellEDIT process.
To offer the most efficient and accurate editing service, preliminary steps involve the onboarding of your cell line and injection optimization; these are crucial prerequisites before initiating the CellEDIT workflow. Once we have familiarized ourselves with the unique characteristics of your cell line, we can then commence the CellEDIT process.
To offer the most efficient and accurate editing service, preliminary steps involve the onboarding of your cell line and injection optimization; these are crucial prerequisites before initiating the CellEDIT workflow. Once we have familiarized ourselves with the unique characteristics of your cell line, we can then commence the CellEDIT process.
To offer the most efficient and accurate editing service, preliminary steps involve the onboarding of your cell line and injection optimization; these are crucial prerequisites before initiating the CellEDIT workflow. Once we have familiarized ourselves with the unique characteristics of your cell line, we can then commence the CellEDIT process.
To offer the most efficient and accurate editing service, preliminary steps involve the onboarding of your cell line and injection optimization; these are crucial prerequisites before initiating the CellEDIT workflow. Once we have familiarized ourselves with the unique characteristics of your cell line, we can then commence the CellEDIT process.
To offer the most efficient and accurate editing service, preliminary steps involve the onboarding of your cell line and injection optimization; these are crucial prerequisites before initiating the CellEDIT workflow. Once we have familiarized ourselves with the unique characteristics of your cell line, we can then commence the CellEDIT process.
To offer the most efficient and accurate editing service, preliminary steps involve the onboarding of your cell line and injection optimization; these are crucial prerequisites before initiating the CellEDIT workflow. Once we have familiarized ourselves with the unique characteristics of your cell line, we can then commence the CellEDIT process.
To offer the most efficient and accurate editing service, preliminary steps involve the onboarding of your cell line and injection optimization; these are crucial prerequisites before initiating the CellEDIT workflow. Once we have familiarized ourselves with the unique characteristics of your cell line, we can then commence the CellEDIT process.
Pascal holds a doctoral degree from ETH Zurich. His passion for entrepreneurship and Startups has led him to the foundation of Cytosurge. He currently serves as CEO of the company and is responsible for all operational and strategic matters of the company.
When away from work, Pascal enjoys spending time at the Rhine river - in summer and in winter. He loves scuba diving and has a passion for the latest tech gadgets.
Management TeamFoundersStephan is a software architect with over 14 years of experience. As one of the company's first employees he has a profound understanding of the FluidFM technology. In his role as CTO he leads the research and development of our solutions and is responsible for the direction, design and development of Cytosurge's products.
When the sun is shining, Stephan enjoys playing soccer. However, he takes far more pleasure in watching soccer matches from inside a stadion or from the comfort of his living room couch.
Management TeamResearch & DevelopmentPablo is a trained micro- and nanosystems engineer and won the ETH medal for his outstanding PhD thesis on FluidFM. With a good connection to our customers he brings invaluable experience into our team. In his position, Pablo ensures that our solutions fully meet our customers' needs and expectations.
To relax, he enjoys video games as well as long talks with his family and friends while sipping a glass of wine.
Management TeamResearch & DevelopmentPascal holds a doctoral degree from ETH Zurich. His passion for entrepreneurship and Startups has led him to the foundation of Cytosurge. He currently serves as CEO of the company and is responsible for all operational and strategic matters of the company.
When away from work, Pascal enjoys spending time at the Rhine river - in summer and in winter. He loves scuba diving and has a passion for the latest tech gadgets.
Management TeamFoundersStephan is a software architect with over 14 years of experience. As one of the company's first employees he has a profound understanding of the FluidFM technology. In his role as CTO he leads the research and development of our solutions and is responsible for the direction, design and development of Cytosurge's products.
When the sun is shining, Stephan enjoys playing soccer. However, he takes far more pleasure in watching soccer matches from inside a stadion or from the comfort of his living room couch.
Management TeamResearch & DevelopmentPablo is a trained micro- and nanosystems engineer and won the ETH medal for his outstanding PhD thesis on FluidFM. With a good connection to our customers he brings invaluable experience into our team. In his position, Pablo ensures that our solutions fully meet our customers' needs and expectations.
To relax, he enjoys video games as well as long talks with his family and friends while sipping a glass of wine.
Management TeamResearch & DevelopmentPascal holds a doctoral degree from ETH Zurich. His passion for entrepreneurship and Startups has led him to the foundation of Cytosurge. He currently serves as CEO of the company and is responsible for all operational and strategic matters of the company.
When away from work, Pascal enjoys spending time at the Rhine river - in summer and in winter. He loves scuba diving and has a passion for the latest tech gadgets.
Management TeamFoundersStephan is a software architect with over 14 years of experience. As one of the company's first employees he has a profound understanding of the FluidFM technology. In his role as CTO he leads the research and development of our solutions and is responsible for the direction, design and development of Cytosurge's products.
When the sun is shining, Stephan enjoys playing soccer. However, he takes far more pleasure in watching soccer matches from inside a stadion or from the comfort of his living room couch.
Management TeamResearch & DevelopmentPablo is a trained micro- and nanosystems engineer and won the ETH medal for his outstanding PhD thesis on FluidFM. With a good connection to our customers he brings invaluable experience into our team. In his position, Pablo ensures that our solutions fully meet our customers' needs and expectations.
To relax, he enjoys video games as well as long talks with his family and friends while sipping a glass of wine.
Management TeamResearch & Development