Direct intra-nuclear CRISPR delivery with FluidFM®
Quantifiable & highly gentle
By delivering CRISPR complexes directly into the nucleus of a specific cell, FluidFM offers a unique in vitro solution to improve the efficiency and applicability of CRISPR across a variety of cell types.
No harsh transfection methods or complex vector designs are required when using direct intra-nuclear injection with FluidFM.
Optimize HDR efficiency and off-target effects by measuring the precise amount of RNP and repair template molecules that are delivered into the nucleus.
The injection procedure is very gentle and maintains high cell viability.
Neuron expressing GFP 24 h after injection of a plasmid encoding GFP using FluidFM. Courtesy of Sen Yan, Jinan University, Guangzhou, China.
Measured volumes of fluorescent CRISPR-Cas9 complexes after injection into mouse primary hepatocytes.
Human Dermal Fibroblast injected with GFP mRNA. 70% of injected cells expressed GFP 24h after injection.