LUTHOR HD: Pushing the boundaries of single cell transcriptome analysis by in vitro mRNA amplification
- Single-cell transcriptome analysis faces limitations due to low input RNA and the dominance of highly expressed transcripts.
- The LUTHOR HD kit, based on the THOR amplification method, differs from traditional library preparation approaches by amplifying RNA directly from mRNA templates isolated from the cell. This linear amplification method enhances the capture of low-copy number transcripts, involving primer annealing, antisense RNA generation, and cDNA transcription for amplification.
- The LUTHOR HD kit successfully detects thousands of genes even with minimal input RNA, both from sRNA solution and single cells, reduces dropouts and improves gene expression resolution.