CellEDIT uses vector free CRISPR delivery to bypass membrane and cytoplasmic barriers, enabling high throughputs edits directly inside the nucleus of single cells.
FluidFM® OMNIUM platform for single cell manipulation
Vector Free Gene Editing for Hard to Transfect Cell Lines
Traditional methods introduce vectors or chemical reagents into cells, triggering stress responses and limiting editing efficiency.
CellEDIT overcomes these barriers with vector free technology that uses patented FluidFM for direct intra nuclear delivery of CRISPR RNPs into single cells.
This approach bypasses membrane and cytoplasmic barriers, enabling precise editing in cell lines that resist standard transfection.
Your Cell Line Profile — Our Edits
iPSC Editing
Precise single cell CRISPR delivery for high viability iPSC editing with validated clonal isolation and sequencing.
Multiplex Gene Editing
Simultaneous targeting of multiple loci through direct intra nuclear CRISPR delivery with high clonal recovery rates.
Customized Knock ins
High fidelity integration of HDR fragments together with CRISPR RNPs to produce knock ins in challenging cell lines using vector free single cell delivery.
Why Choose CellEDIT?
CellEDIT combines vector free CRISPR delivery with single cell control to enable reliable editing in cell lines that resist conventional transfection.
Enable editing in hard-to-transfect cells:
Successfully modify challenging lines that resist conventional transfection using direct FluidFM-based RNP delivery.
Preserve cell viability:
Maintain viable, functional clones thanks to gentle nanoinjection that avoids harsh chemical or viral transfection conditions.
Minimize off-target effects:
Deliver pre-assembled RNPs directly into the nucleus to reduce unintended edits and improve the quality of your engineered cell lines.
Ensure clonal purity:
Build your cell lines from a true single-cell workflow, enabling monoclonal origin without extensive heterogeneous population screening.
The CellEDIT Workflow
Key Benefits:
- Precision nanoinjection ensures high editing efficiency, reduced off-target effects, and cell viability, generating custom monoclonal cell lines in about 10 weeks.
- Includes gRNA design, two edited clones plus wild-type controls, full reports with sequencing and monoclonality proof.
- Ideal for biopharma research, therapeutic development, and synthetic biology.
CRISPR Gene Editing Workflow:
- CRISPR Design: Editing strategy development.
- Cell Plating: Automated single-cell isolation for cell growth.
- CRISPR Injection: Injecting CRISPR RNPs into all cells.
- Singulation & Outgrowth: Cells grow and form colonies.
- Screening: Analyzing targeted genetic sequences.
- Quality Control: Ensuring cell monoclonality and checking for mycoplasma.
Take Advantage of our Winter Deals —
CellEDIT delivers vector free CRISPR editing in challenging cell lines with rapid turnaround from design to validated monoclonal clones.