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Updates from the Cytosurge Applications team
Done
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Apero and poster presentation
Done
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Registration and coffee
Done
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Closing Note
Done
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Round table discussion
Done
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Accelerated generation of gene-engineered monoclonal CHO cell lines using FluidFM nanoinjection and CRISPR/Cas9 - Session Gene engineering and CRISPR
Dr. Justin AnthonyDone
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Coffee break
Done
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The SARS-COV-2 cytoplasmic tail story: Biophysical Approach in the understanding of SARS-CoV tropism
Bhanupriya PanigrahiDone
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Implementing Live-seq on a triple-negative breast cancer cell line - Session: Single-cell RNA sequencing: Live-seq and biopsies
Margot Le-BotDone
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Genome editing in single cells: Measurement considerations for emerging technologies
Dr. Simona PatangeDone
PhD student, ELKH EK MFA Nanobiosensorics Lab, Budapest, Hungary
Kinga Dóra Kovács kovacs.kinga.dora@ek.hun-ren.hu
HUN-REN Centre for Energy Research, Hungary
Abstract:
In the past decade, extracellular vesicles (EVs) have attracted substantial interest in biomedicine. With progress in the field, we have an increasing understanding of cellular responses to EVs. In this Technical Report, we describe the direct nanoinjection of EVs into the cytoplasm of single cells of different cell lines. By using robotic fluidic force microscopy (robotic FluidFM), nanoinjection of GFP positive EVs and EV-like particles into single live HeLa, H9c2, MDA-MB-231 and LCLC-103H cells proved to be feasible. This injection platform offered the advantage of high cell selectivity and efficiency. The nanoinjected EVs were initially localized in concentrated spot-like regions within the cytoplasm. Later, they were transported towards the periphery of the cells. Based on our proof-of-principle data, robotic FluidFM is suitable for targeting single living cells by EVs and may lead to information about intracellular EV cargo delivery at a single-cell level.
Kovács, K.D., Visnovitz, T., Gerecsei, T., Peter, B., Kurunczi, S., Koncz, A., Németh, K., Lenzinger, D., Vukman, K. V., Balogh, A., Rajmon, I., Lőrincz, P., Székács, I., Buzás, E. I., & Horvath, R. (2023). Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy. Journal of Extracellular Vesicles, 12, e12388.