FluidFM Micropipette
Specifications
Material: | Silicon Nitride (SiN) |
Cantilever Dimensions: Inside height: | 200 x 36 x 1.7 µm (LxWxH) 1500 nm for 4 N/m ,1000 nm for 2 N/m and 500 nm for 1 N/m and 0.3 N/m |
Specified Spring Constant: | 0.3 N/m, 2 N/m or 4 N/m, 4 N/m only with 8 µm apertures |
| Opening Type: | Circular, flat |
Aperture Size: | 2 µm, 4 µm or 8 µm |
Reflective Coating: | Cr/Au (40 nm) |
FAQ
What are the typical flows and volumes involved when working with FluidFM probes?
The flow of water through a FluidFM cantilever is typically in the range of fl/s to pL/s. This is 1000x below even the best flow sensors, which have detection limits of around 1 nL/s. Accordingly the dispensed volumes start at single fL for applications such as spotting in air or injecting cells.
How long can I use the same FluidFM probe?
The lifetime of a FluidFM probe depends greatly on the experimental conditions, as well as on the applied filling, cleaning and storage protocols. Thus, a FluidFM probe’s lifetime varies between one experiment (a few hours) and one month. Please contact us for advice on the optimal probe protocols for your experiment.
selected micropipette applications
Single Cell
Adhesion
Cells bind to various surfaces and substrates. The exact measurement of the strength and characteristics of these bonds provide valuable information for numerous biological and clinical research topics.
Colloidal spectroscopy
Interactive forces between colloidal particles and other surfaces can be measure directly with FluidFM. A spherical colloid is the ideal probe for local elasticity measurement of complex substrates.
