FluidFM® ADD‑ON for Bruker & JPK AFMs
Empower your Bruker or JPK atomic force microscope with new applications.
Extend the application scope of your Bruker or JPK atomic force microscope with our FluidFM ADD-ON. The FluidFM ADD-ON is compatible with the Bruker BioScope Resolve AFM and all JPK NanoWizard®, ForceRobot® and CellHesion® AFMs.
Full integration
Software integration of FluidFM into AFM software
Full compatibility
Sader-based cantilever calibration fully functional for FluidFM probes
Increase throughput
10x higher throughput for cell adhesion measurements with FluidFM
Easy & fast probe mounting
The mounting of a FluidFM probe onto your AFM is fast and simple and does not require any tooling. The video shows the mounting onto a JPK NanoWizard AFM.
Video courtesy of Bruker.
Safe & reliable
The custom FluidFM probe holder for your AFM assures safe, tight and reliable mounting of FluidFM probes without the need of any tweezers.
Tailored to each system
Our FluidFM probe adapters are tailored to the geometrical and physical requirements of the specific JPK and Bruker AFMs.
Use Cases with a FluidFM ADD-ON and Bruker & JPK AFMs
Mechanobiology
Original video length ~10 min. Video courtesy of Bruker.
Quantification of cell - surface interaction
The interaction of a H431 cell is being quantified against the surface on a 8x8 array. The experiment was conducted with a Bruker BioScope Resolve and a FluidFM micropipette.
Pick up of a bead for force mapping on living cells
A colloid is picked up by a FluidFM probe and used to measure a force map on a living cell. The FluidFM microfluidic control is fully integrated into the AFM software.
Original video length ~9 min. Video courtesy of Bruker.
The bead is held with -400 mbar while the measurement takes place. The video shows a full force map cycle.
Phase contrast image of living vero cells, the FluidFM micropipette and 11 µm polystyrene bead before (a), during (b) and after pickup (c). (d) Overlay of optical image with Height map using the polystyrene bead. (e, f) Height and apparent stiffness map of two cells.
Image courtesy of Bruker.
Nanoprinting
Video courtesy of Bruker.
Creating droplet arrays from fL to nL
In this video the letters JPK are spotted onto a glass dish in air. These femtoliter sized water droplets quickly evaporate under ambient conditions. For longer lasting droplets a bit of glycerol can be added to the ink. However, often it is desired that only the proteins or nanoparticle payload stays on the sample, while the carrier solution evaporates.
Reproducibility of FluidFM printed spots
3x4 spots of 30 nm gold nanoparticles are deposited with a FluidFM probe
They are later scanned with the same probe
The reproducibility of all 12 spots is very high
The slope channel is extremely useful to calculate the spot area and volume
The 30 nm Au nano particles are individual or aggregated, and typically slightly to the edge of the spot
Image courtesy of Bruker.
The height and slope channel of 30nm Au nanoparticles, printed with a FluidFM nanopipette.
Optical image of the deposited spots; 2x2 maps with 3x3 µm2, gap 1.5 µm.
Image courtesy of Bruker.
Print & scan with the same probe
AFM scans are also possible with the FluidFM nanopipette used for printing. Yet doubled features occur due to the tip geometry, which features corners around the opening. 2 of which at the contact level. These doubled feature effect can be avoided by tilting the AFM.
First structure: Motors A/B/C = 0 µm
Second structure: Motors A/B = 700 µm; C = 0 µm
Images courtesy of Bruker.
Applications using injection
Video courtesy of Bruker.
Injecting into a single cell
Typical injected volumes range from a few dozen to 200 fL, a small fraction of the overall cell volume of 4 to 5 pL. If needed the injected volume can be quantified with fL precision for each injected cell with a fluorescence intensity based approach.
In the video, four cells are injected with propidium iodide, which stains the DNA fluorescently. It is common that any payload e.g. CRISPR is co-injected with a fluorescent tracer to have a live feedback on both the injection success and the injected volume.