FluidFM® ADD‑ON for Bruker & JPK AFMs

Empower your Bruker or JPK atomic force microscope with new applications.

JPK AFM with a FluidFM ADD-ON

Bruker & JPK BioAFM logo

Extend the application scope of your Bruker or JPK atomic force microscope with our FluidFM ADD-ON. The FluidFM ADD-ON is compatible with the Bruker BioScope Resolve AFM and all JPK NanoWizard®, ForceRobot® and CellHesion® AFMs.

Full integration

Software integration of FluidFM into AFM software

Full compatibility

Sader-based cantilever calibration fully functional for FluidFM probes

Increase throughput

10x higher throughput for cell adhesion measurements with FluidFM

Empower your research with FluidFM

Easy & fast probe mounting

The mounting of a FluidFM probe onto your AFM is fast and simple and does not require any tooling. The video shows the mounting onto a JPK NanoWizard AFM.


Video courtesy of Bruker.

JPK NanoWizard with a FluidFM probe

Safe & reliable

The custom FluidFM probe holder for your AFM assures safe, tight and reliable mounting of FluidFM probes without the need of any tweezers.

Tailored to each system

Our FluidFM probe adapters are tailored to the geometrical and physical requirements of the specific JPK and Bruker AFMs.

FluidFM probes for Bruker and JPK AFMs

Product Note

Learn more about the integration of FluidFM functionality into JPK BioAFM products.

Use Cases with a FluidFM ADD-ON and Bruker & JPK AFMs



Original video length ~10 min. Video courtesy of Bruker.

Quantification of cell - surface interaction

The interaction of a H431 cell is being quantified against the surface on a 8x8 array. The experiment was conducted with a Bruker BioScope Resolve and a FluidFM micropipette.

Pick up of a bead for force mapping on living cells

A colloid is picked up by a FluidFM probe and used to measure a force map on a living cell. The FluidFM microfluidic control is fully integrated into the AFM software. 


Original video length ~9 min. Video courtesy of Bruker.

The bead is held with -400 mbar while the measurement takes place. The video shows a full force map cycle.

Phase contrast image of living vero cells, the FluidFM micropipette and 11 µm polystyrene bead before (a), during (b) and after pickup (c). (d) Overlay of optical image with Height map using the polystyrene bead. (e, f) Height and apparent stiffness map of two cells.

FluidFM Mechanobiology - Colloidal spectroscopy (Image courtesy of Bruker)

Image courtesy of Bruker.



Video courtesy of Bruker.

Creating droplet arrays from fL to nL

In this video the letters JPK are spotted onto a glass dish in air. These femtoliter sized water droplets quickly evaporate under ambient conditions. For longer lasting droplets a bit of glycerol can be added to the ink. However, often it is desired that only the proteins or nanoparticle payload stays on the sample, while the carrier solution evaporates.

Reproducibility of FluidFM printed spots

  • 3x4 spots of 30 nm gold nanoparticles are deposited with a FluidFM probe

  • They are later scanned with the same probe

  • The reproducibility of all 12 spots is very high

  • The slope channel is extremely useful to calculate the spot area and volume

  • The 30 nm Au nano particles are individual or aggregated, and typically slightly to the edge of the spot

FluidFM Nanoprinting - Spotting (Image courtesy of Bruker)

Image courtesy of Bruker.

The height and slope channel of 30nm Au nanoparticles, printed with a FluidFM nanopipette.

Optical image of the deposited spots; 2x2 maps with 3x3 µm2, gap 1.5 µm.

FluidFM Nanoprinting - Spotting (Image courtesy of Bruker)

Image courtesy of Bruker.

FluidFM nanopipette

Print & scan with the same probe

AFM scans are also possible with the FluidFM nanopipette used for printing. Yet doubled features occur due to the tip geometry, which features corners around the opening. 2 of which at the contact level. These doubled feature effect can be avoided by tilting the AFM.

First structure: Motors A/B/C = 0 µm

Second structure: Motors A/B = 700 µm; C = 0 µm

FluidFM Nanoprinting - Printed lines without tilting AFM (Image courtesy of Bruker)


Visualization of a tilted AFM (Image courtesy of Bruker)
FluidFM Nanoprinting - Printed lines with tilting AFM (Image courtesy of Bruker)

Images courtesy of Bruker.

Applications using injection


Video courtesy of Bruker.

Injecting into a single cell

Typical injected volumes range from a few dozen to 200 fL, a small fraction of the overall cell volume of 4 to 5 pL. If needed the injected volume can be quantified with fL precision for each injected cell with a fluorescence intensity based approach.

In the video, four cells are injected with propidium iodide, which stains the DNA fluorescently. It is common that any payload e.g. CRISPR is co-injected with a fluorescent tracer to have a live feedback on both the injection success and the injected volume.

Would you like to discuss your experiment with our FluidFM experts?

Contact us now and we will help you to empower your research with FluidFM.