FluidFM® ADD‑ON for Bruker & JPK AFMs

To extend the capability of your Bruker AFM with FluidFM ADD-ON:

JPK AFM with a FluidFM ADD-ON

Bruker & JPK BioAFM logo

Extend the application scope of your Bruker or JPK atomic force microscope with our FluidFM ADD-ON. The FluidFM ADD-ON is compatible with the Bruker BioScope Resolve AFM and JPK NanoWizard®, ForceRobot® and CellHesion® AFMs.

Safe & reliable

The custom FluidFM probe holder for your AFM assures safe, tight and reliable mounting of FluidFM probes without the need of any tweezers.

JPK NanoWizard with a FluidFM probe

Video courtesy of Bruker.

Easy & fast probe mounting

The mounting of a FluidFM probe onto your AFM is fast and simple and does not require any tooling. The video shows the mounting onto a JPK NanoWizard AFM.

Tailored to each system

Our FluidFM probe adapters are tailored to the geometrical and physical requirements of the specific JPK and Bruker AFMs.

FluidFM probes for Bruker and JPK AFMs

Are you looking for a standalone platform suited for Life Sciences application?

Use Cases with a FluidFM ADD-ON and Bruker & JPK AFMs



Original video length ~10 min. Video courtesy of Bruker.

Quantification of cell - surface interaction

The interaction of a H431 cell is being quantified against the surface on a 8x8 array. The experiment was conducted with a Bruker BioScope Resolve and a FluidFM micropipette.

Pick up of a bead for force mapping on living cells

A colloid is picked up by a FluidFM probe and used to measure a force map on a living cell. The FluidFM microfluidic control is fully integrated into the AFM software. 

The bead is held with -400 mbar while the measurement takes place. The video shows a full force map cycle.


Original video length ~9 min. Video courtesy of Bruker.

FluidFM Mechanobiology - Colloidal spectroscopy (Image courtesy of Bruker)

Image courtesy of Bruker. Phase contrast image of living vero cells, the FluidFM micropipette and 11 µm polystyrene bead before (a), during (b) and after pickup (c). (d) Overlay of optical image with Height map using the polystyrene bead. (e, f) Height and apparent stiffness map of two cells.



Video courtesy of Bruker.

Creating droplet arrays from fL to nL

In this video the letters JPK are spotted onto a glass dish in air. These femtoliter sized water droplets quickly evaporate under ambient conditions. For longer lasting droplets a bit of glycerol can be added to the ink. However, often it is desired that only the proteins or nanoparticle payload stays on the sample, while the carrier solution evaporates.

Reproducibility of FluidFM printed spots

3x4 spots of 30 nm gold nanoparticles are deposited with a FluidFM probe

  • They are later scanned with the same probe
  • The reproducibility of all 12 spots is very high
  • The slope channel is extremely useful to calculate the spot area and volume
  • The 30 nm Au nano particles are individual or aggregated, and typically slightly to the edge of the spot
FluidFM Nanoprinting - Spotting (Image courtesy of Bruker)

Image courtesy of Bruker. The height and slope channel of 30nm Au nanoparticles, printed with a FluidFM nanopipette.

FluidFM Nanoprinting - Spotting (Image courtesy of Bruker)

Image courtesy of Bruker. Optical image of the deposited spots; 2x2 maps with 3x3 µm2, gap 1.5 µm.

Print & scan with the same probe

AFM scans are also possible with the FluidFM nanopipette used for printing. Yet doubled features occur due to the tip geometry, which features corners around the opening. 2 of which at the contact level. These doubled feature effect can be avoided by tilting the AFM.

First structure: Motors A/B/C = 0 µm

Second structure: Motors A/B = 700 µm; C = 0 µm

FluidFM Nanoprinting - Printed lines without tilting AFM (Image courtesy of Bruker)


Visualization of a tilted AFM (Image courtesy of Bruker)
FluidFM Nanoprinting - Printed lines with tilting AFM (Image courtesy of Bruker)

Images courtesy of Bruker.

Did we spark your interest?