Volume Calculator

How the precise volume quantification works.


Our software-integrated Volume Calculator can precisely quantify the volume injected into each cell. Working with fluorescent compounds, the microfabricated FluidFM nanosyringe serves as a precise reference for calculating the injected volume. The inherent manufacturing precision and the constant dimensions of the FluidFM nanosyringe and its inner channel make it possible for the FluidFM nanosyringe to provide a reference fluorescence intensity per femtoliter. After injection, the fluorescence intensity of each cell is compared to this reference intensity, allowing you to calculate precisely the injected volume for each injected cell. The Volume Calculator guides you step-by-step towards a self-generated report.​

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

The Volume Calculator provides for each injected cell a precise quantification of injected volume.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

Microchannel height of the FluidFM nanosyringe defines the volumetric reference. (Image: transversal cut of the nanosyringe)

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

FluidFM nanosyringe provides a reference fluorescence intensity per femtoliter.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

Easy step-by-step software interface, here results view.