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Live-Seq


Live-seq is a live-cell sequencing workflow that enables time-resolved transcriptome analysis by taking cytoplasmic biopsies from individual cells using a FluidFM Nanosyringe — capturing gene expression snapshots at multiple timepoints without compromising cell viability.

 
Extraction Workflow Overview





Cell line onboarding

Understand your cells and optimize your cell culture for a Live-Seq experiment.



Feasibility of the Biopsy Collection

Ensure that biopsies can be collected with the chosen parameters and conditions without issues.

      
        

Feasibility of the Biopsy viability

Make sure that your cell remains alive after the biopsy.

 


Clean Biopsy Collection

After all preparation and optimization steps, collect biopsies from the target cells.

Viability and Sequential Biopsy Collection


 


Library Preparation



Would you like to plan your experiment with our FluidFM experts?