Select which cell you want –
place it where you want. 

Easy & cell-friendly isolation for suspension & adherent cells.

 Easy procedure

  1. Select a specific cell by mouse click on the screen.

  2. The selected cell is being picked up

  3. Select a new well, dish or other containers.

  4. Release your cell.

  5. Expand selected single cell into a monoclonal line. 

The ideal method to select the cell of your choice for downstream analysis 

Pick the desired cell directly from the culture, with minimal analyte dilution, and transfer it to the downstream analysis step you need.​ Perform for example NGS sequencing, assays, protein chips, ESI MS, Maldi TOF and NMR, or expand the cell into a monoclonal culture. FluidFM isolation is particularly powerful for adherent and suspension cells that are precious and/or rare.​

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

Advantages of a bottom-up approach vs. conventional cell sorting techniques

Start with the needle. No need to find it in the haystack.

High viability

Minimal forces are applied to a cell ensuring minimal stress and leading to cell viability of over 95%.

Selective

Cells can be individually selected, by optically detectable features (phenotype).

Precise

Select a specific cell directly from a culture. Have evidence and traceability of the very selected cell for the monoclonality report.

Select source, select destination. Stand back.


Take advantage of the fully integrated motorized stages and microscope. Select the cells of interest and the destination, then let the system autonomously run the the isolation or sorting process.

Video: Automated isolation of suspension CHO cells. (Video is accelerated, original length approx. 14 min) 


Contact us to learn more

Seed and expand selected cells. 

Cells remain viable and grow unperturbed.


Single selected cells can be transferred by mouse click into a new well and each is expanded into a colony with minimal cellular stress. In this way, you can be certain that each colony originates from one specific single cell.

Cells of interest can be selected based on the expression of a fluorescent marker or simply based on morphological properties.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

1. Isolated CHO cells deposited in a 3x3 array into a new wellplate, imaged just after isolation.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

2. 24h after isolation.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

3. 48h after isolation. Cell growth rate is not impacted by the FluidFM isolation procedure.

Expansion of an isolated CHO cell

Isolated CHO cell growing in a CO2 independent medium and 37°C. The cell was isolated using a FluidFM micropipette with 4 µm aperture. After isolation, the cell was imaged under bright field conditions every 15 minutes directly with the FluidFM BOT BIO Series.

Suspension or adherent cells

Both suspension and adherent cells can directly be picked up from a cell culture and released in a selected container. For adherent cell cultures, trypsin is dispensed locally via the same probe to dissociate specific cells from the substrate.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

Local trypsinization: Additional integrated step for adherent cells.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

     Isolation: Picking the cell & placing it into a new well.

Gateway to single cell omics

- Identify rare cells by their phenotype or fluorescent signal.
- Sort them into other wells (individually or in pools).
- Interface to further downstream analysis at a single cell level.

Discuss your experiment with our experts

For cells and other micro-organisms and objects

Odoo - Sample 1 for three columns

Adherent & suspension cells

Compatible with almost any cell type such as iPSC, mouse or human ESC or primary hepatocytes.

When working with adherent cells, locally deliver trypsin via the same probe to dissociate specific cells from the substrate where they are cultured.

Odoo - Sample 3 for three columns

Microbes

Be it microscopic algae, bacteria, fungi or protozoa; FluidFM can address organisms from a few nanometers to dozens of micrometers in size.

Image courtesy E. Potthoff, ETH Zurich.

Odoo - Sample 2 for three columns

Colloids

FluidFM addresses the largest range of colloid dimensions - from 500 nm up to 100 µm.

Using focused ion beam, special FluidFM probes can be specifically modified so that even smaller or larger particles can potentially be manipulated.

    Selected publications:

    FluidFM's suitability to isolate single cells - whether suspension or adherent, whether mammalian or microbial - has been published:

          Webinar with a live demo:

          FluidFM cell isolation

          • Get to know FluidFM as a tool for single cell research.

          • Understand how FluidFM cell isolation works and how it empowers single cell research.

          • Learn which applications can benefit from FluidFM cell isolation.

          Odoo • Text and Image

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