Select which cell you want –
place it where you want. 

Easy & cell-friendly isolation.

 
 

 Easy procedure

  1. Select a specific cell by mouse click on the screen.

  2. The selected cell is being picked up

  3. Select a new well, dish or other containers.

  4. Release your cell.

  5. Expand your cell.

Seed and expand selected cells.

Single selected cells can be transferred by mouse click into a new well and each is expanded into a colony with minimal stress.

In this way, you can be certain that each colony originates from one specific single cell.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

Established colonies of CHO cells shown in a 12-well plate 48 hours after isolation.

 

 

Expansion of an isolated CHO cell

Isolated CHO cell growing in a CO2 independent medium and 37°C. The cell was isolated using a FluidFM micropipette with 4 µm aperture. After isolation, the cell was imaged under bright field conditions every 15 minutes directly with the FluidFM BOT BIO Series. 

Advantages of the bottom-up approach vs. conventional cell sorting techniques

High viability

Minimal forces are applied to a cell ensuring minimal stress and leading to cell viability of over 95%.

Selective

Cells can be individually selected, by optically detectable features (phenotype).

Precise

Select a specific cell directly from a culture. Have evidence and traceability of the very selected cell for the monoclonality report.

Suspended or adherent cells

Both suspended and adherent cells can directly be picked up from a cell culture and released in a selected container. For adherent cell cultures, trypsin is dispensed locally via the same probe to dissociate specific cells from the substrate.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

Local trypsinization: Additional integrated step for adherent cells.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

     Isolation: Picking the cell & placing it into a new well.

Gateway to single cell omics

- Identify rare cells by their phenotype or fluorescent signal.
- Sort them into other wells (individually or in pools).
- Interface to further downstream analysis at a single cell level.

Discuss your experiment with our experts

For cells and other micro-organisms and objects

Odoo - Sample 1 for three columns

Adherent & suspended cells

Compatible with almost any cell type such as iPSC, mouse or human ESC or primary hepatocytes.

When working with adherent cells, locally deliver trypsin via the same probe to dissociate specific cells from the substrate where they are cultured.

Odoo - Sample 3 for three columns

Microbes

Be it microscopic algae, bacteria, fungi or protozoa; FluidFM can address organisms from a few nanometers to dozens of micrometers in size.

Image courtesy E. Potthoff, ETH Zurich.

Odoo - Sample 2 for three columns

Colloids

FluidFM addresses the largest range of colloid dimensions - from 500 nm up to 100 µm.

Using focused ion beam, special FluidFM probes can be specifically modified so that even smaller or larger particles can potentially be isolated.

          Webinar with a live demo:

          FluidFM cell isolation

          • Get to know FluidFM as a tool for single cell research.

          • Understand how FluidFM cell isolation works and how it empowers single cell research.

          • Learn which applications can benefit from FluidFM cell isolation.

          Odoo • Text and Image

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